Nucleic Acids Research, 1995, Vol. 23, No. 22 4670-4676
© 1995
Articles |
The Xenopus intron-encoded U17 snoRNA is produced by exonucleolytic processing of its precursor in oocytes
Dipatimento di Biologia, Università di Roma ‘Tor Vergata’ 1–00133 Rome, Italy 1Dipartimento di Biologia, Terza Università di Roma 1–00154 Rome, Italy
*To whom correspondence should be addressed
Received July 28, 1995. Accepted October 13, 1995.
U17 is a small nucleolar RNA encoded in the Introns of the Xenopus laevis gene for ribosomal protein S7 (formerly S8, see Note). To study the mechanisms involved In Its in vivo processing from S7 transcripts, various In vitro synthesized RNAs embedding a U17 sequence have been mlcroin)ected into the germinal vesicle of Xenopus oocytes and their processing analysed. In particular, the Xenopus U17gene copies a and f and a U17 gene copy from the pufferflsh Fugu rubripes have been used. Information about the nature of the processing activities involved in U17 RNA maturation have been sought by Injecting transcripts protected from exonucleolytic attack at their 5'-end by capping and/or lengthened at their 3'-end by poiyadenylation. The results obtained indicate that U17 RNA processing is a spllcing-independent event and that it is mostly or entirely due to exonucleolytic degradation at both the 5'- and 3'-ends of the precursor molecules. Moreover, it is concluded that the enzymes involved are of the processive type. It is suggested that the apparatus for U17 RNA processing is that responsible for the degradation of all excised and debranched introns. Protection from exonucleolytic attack, due to the tight structure and/or to the binding of specific proteins, would be the mechanism by which U17 RNA Is produced.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. S. Mattick and M. J. Gagen Review ArticleThe Evolution of Controlled Multitasked Gene Networks: The Role of Introns and Other Noncoding RNAs in the Development of Complex Organisms Mol. Biol. Evol., September 1, 2001; 18(9): 1611 - 1630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Darzacq and T. Kiss Processing of Intron-Encoded Box C/D Small Nucleolar RNAs Lacking a 5',3'-Terminal Stem Structure Mol. Cell. Biol., July 1, 2000; 20(13): 4522 - 4531. [Abstract] [Full Text]
|
|
|
|
|
|
T. Villa, F. Ceradini, and I. Bozzoni Identification of a Novel Element Required for Processing of Intron-Encoded Box C/D Small Nucleolar RNAs in Saccharomyces cerevisiae Mol. Cell. Biol., February 15, 2000; 20(4): 1311 - 1320. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. S. Lange, M. Ezrokhi, F. Amaldi, and S. A. Gerbi Box H and Box ACA Are Nucleolar Localization Elements of U17 Small Nucleolar RNA Mol. Biol. Cell, November 1, 1999; 10(11): 3877 - 3890. [Abstract] [Full Text]
|
|
|
|
|
|
L.-H. Qu, A. Henras, Y.-J. Lu, H. Zhou, W.-x. Zhou, Y.-Q. Zhu, J. Zhao, Y. Henry, M. Caizergues-Ferrer, and J.-P. Bachellerie Seven Novel Methylation Guide Small Nucleolar RNAs Are Processed from a Common Polycistronic Transcript by Rat1p and RNase III in Yeast Mol. Cell. Biol., February 1, 1999; 19(2): 1144 - 1158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Pelczar and W. Filipowicz The Host Gene for Intronic U17 Small Nucleolar RNAs in Mammals Has No Protein-Coding Potential and Is a Member of the 5'-Terminal Oligopyrimidine Gene Family Mol. Cell. Biol., August 1, 1998; 18(8): 4509 - 4518. [Abstract] [Full Text]
|
|
|
|
|
|
E. Petfalski, T. Dandekar, Y. Henry, and D. Tollervey Processing of the Precursors to Small Nucleolar RNAs and rRNAs Requires Common Components Mol. Cell. Biol., March 1, 1998; 18(3): 1181 - 1189. [Abstract] [Full Text]
|
|
|
|
 |
|
 C Crosio, F Cecconi, P Mariottini, G Cesareni, S Brenner, and F Amaldi Fugu intron oversize reveals the presence of U15 snoRNA coding sequences in some introns of the ribosomal protein S3 gene. Genome Res., December 1, 1996; 6(12): 1227 - 1231. [Abstract] [PDF]
|
|