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Nucleic Acids Research, 1995, Vol. 23, No. 3 427-433
© 1995

MOLECULAR BIOLOGY

Homodimer of p50 (NF{kappa}B1) does not introduce a substantial directed bend into DNA according to three different experimental assays

Dmitry V. Kuprash1,2,3, Nancy R. Rice3 and Sergei A. Nedospasov1,2,*

1Laboratory of Cytokine Molecular Biology, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences Moscow 117984, Russia 2Biological Carcinogenesis and Development Program, Program Resources, Inc./DynCorp., Laboratory of Molecular Immunoregulation, Biological Response Modifiers Program, National Cancer Institute—Frederick Cancer Research and Development Center Frederick, MD 21702-1201, USA 3ABL-Basic Research Program, National Cancer Institute—Frederick Cancer Research and Development Center Frederick, MD 21702-1201, USA

*To whom correspondence should be addressed at: BCDP, PRI/DynCorp, National Cancer Institute, Frederick Cancer Research and Development Center, Building 560, Room 31-70, Frederick, MD 21702-1201, USA

Received October 12, 1994. Revised November 23, 1994. Accepted November 23, 1994.

Transcription factors can distort the conformation of the DNA double helix upon binding to their target sites. Previously, studies utilizing circular permutation—electrophoretlc mobility shift assay suggested that the homodimer of p50 (NF{kappa}B1), canonical NF-{kappa}B (p65-p50), as well as several non-canonical NF-{kappa}B/Rel complexes, may induce substantial DNA bending at the binding site. Here we have applied three additional experimental approaches, helical phasing analysis, mlniclrcle binding and cyclization kinetics, and conclude that the homodimer of p50 introduces virtually no directed bend into the consensus {kappa}B sequences GGGACTTTCC or GGGAATTCCC.


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