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Nucleic Acids Research, 1995, Vol. 23, No. 3 485-490
© 1995

MOLECULAR BIOLOGY

Intra-chromosomal rearrangements generated by Cre-lox site-specific recombination

Scott L. Medberry, Emily Dale+, Minmin Qin and David W. Ow*

Plant Gene Expression Center, US Department of Agriculture 800 Buchanan Street, Albany, CA 94710, USA and Department of Plant Biology, University of California Berkeley, CA 94720, USA

*To whom correspondence should be addressed

Received September 16, 1994. Revised December 2, 1994. Accepted December 2, 1994.

Chromosomal rearrangements are useful genetic and breeding tools but are often difficult to detect and characterize. To more easily identify and define chromosome deletions and inversions, we have used the bacteriophage P1 Cre-loxsite-specific recombination system to generate these events in plants. This involves three steps: (i) the introduction of two lox sites into one locus in a plant genome, including one site within a modified Ds transposon; (ii) Ac transposase-mediated transposition of the Ds-lox element to a new locus on the same chromosome; (iii) Cre-mediated site-specific recombination between the two lox sites that bracket a chromosome segment. We report the production of a deletion and three inversion events in tobacco. The utility of chromosomal segments bracketed by lox sites for targeted manipulation and cloning is discussed.

+Present address Lawrence Berkeley Laboratories, I Cyclotron Road, Building 74. Berkeley, CA 94720, USA


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