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Nucleic Acids Research, 1995, Vol. 23, No. 5 742-744
© 1995

ENZYMOLOGY

Sse8647l, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'

Yoshizumi Ishino*, Yosiko Nomura and Ikunoshin Kato

Biotechnology Research Laboratories, Takara Shuzo Co. Ltd. Seta 3-4-1, Otsu, Shiga 520-21, Japan

*To whom correspondence should be addressed

Received December 5, 1994. Revised January 10, 1995. Accepted January 10, 1995.

We Isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Steptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave {lambda} phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and {varphi}X174 DNAs.


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