Skip Navigation

This Article
Right arrow Print PDF (3148K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrowRequest Permissions
Citing Articles
Right arrowScopus Links
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Beaudry, D.
Right arrow Articles by Perreault, J.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beaudry, D.
Right arrow Articles by Perreault, J.-P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 5 745-752
© 1995

RNA

The RNA of both polarities of the peach latent mosaic viroid self-cleaves in vitro solely by single hammerhead structures

Danlèle Beaudry, Frédéric Bussière, François Lareau, Carl Lessard and Jean-Pierre Perreault*

Département de Biochimie Université de Sherbrooke Sherbrooke, Québec J1H 5N4, Canada

*To whom correspondence should be addressed

Received December 2, 1994. Revised January 20, 1995. Accepted January 20, 1995.

Hammerhead self-cleavage of dimeric, monomeric, truncated and mutated transcripts derived from both polarities of the peach latent mosaic viroid (PLMVd) were characterized. In contrast to some results previously published for a very close sequence variant (see ref. 1), these RNAs exhibit a virtually identical self-cleavage during transcription and after purification. By self-cleavage of dimeric transcripts with normal and mutated hammerhead domains and by complementation experiments, we show that the cleavage reactions Involve only single hammerhead structures. This observation contrasts with the case of avocado sunblotch viroid (ASBVd), the other self-cleaving viroid, whose mechanism involves mostly double hammerhead structures, whereas single hammerhead cleavage is associated with viroid-like plant satellite RNAs. The difference in stability between the native secondary structures adopted by viroids and the autocatalytic structures, including the hammerheadmotif, governs the efficiency of the self-cleavage reaction. The transition between these conformers is the limiting step In catalysis and is related exclusively to the left arm region of PLMVd secondary structure, which includes the hammerheadsequences. Most of the mutations between the variant we used and the sequence variant previously published are located in this left arm region, which may explain to a great extent the differences in their cleavage efficiency. No interactions with long-range sequences contributing to the autocatalytic tertiary structure were revealed in these experiments.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
A. Dube, T. Baumstark, M. Bisaillon, and J.-P. Perreault
The RNA strands of the plus and minus polarities of peach latent mosaic viroid fold into different structures
RNA, March 1, 2010; 16(3): 463 - 473.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Delgado, A. E. Martinez de Alba, C. Hernandez, and R. Flores
A Short Double-Stranded RNA Motif of Peach Latent Mosaic Viroid Contains the Initiation and the Self-Cleavage Sites of Both Polarity Strands
J. Virol., October 15, 2005; 79(20): 12934 - 12943.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
P. Landry and J.-P. Perreault
Identification of a Peach Latent Mosaic Viroid Hairpin Able To Act as a Dicer-Like Substrate
J. Virol., May 15, 2005; 79(10): 6540 - 6543.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
F. Cote, D. Levesque, and J.-P. Perreault
Natural 2',5'-Phosphodiester Bonds Found at the Ligation Sites of Peach Latent Mosaic Viroid
J. Virol., January 1, 2001; 75(1): 19 - 25.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. A. Rojas, A. Vazquez-Tello, G. Ferbeyre, F. Venanzetti, L. Bachmann, B. Paquin, V. Sbordoni, and R. Cedergren
Hammerhead-mediated processing of satellite pDo500 family transcripts from Dolichopoda cave crickets
Nucleic Acids Res., October 15, 2000; 28(20): 4037 - 4043.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
F. Bussiere, J. Ouellet, F. Cote, D. Levesque, and J. P. Perreault
Mapping in Solution Shows the Peach Latent Mosaic Viroid To Possess a New Pseudoknot in a Complex, Branched Secondary Structure
J. Virol., March 15, 2000; 74(6): 2647 - 2654.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
F. Bussiere, J. Lehoux, D. A. Thompson, L. J. Skrzeczkowski, and J.-P. Perreault
Subcellular Localization and Rolling Circle Replication of Peach Latent Mosaic Viroid: Hallmarks of Group A Viroids
J. Virol., August 1, 1999; 73(8): 6353 - 6360.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. A. James and I. Gibson
The Therapeutic Potential of Ribozymes
Blood, January 15, 1998; 91(2): 371 - 382.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Navarro and R. Flores
Chrysanthemum chlorotic mottle viroid: Unusual structural properties of a subgroup of self-cleaving viroids with hammerhead ribozymes
PNAS, October 14, 1997; 94(21): 11262 - 11267.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.