Nucleic Acids Research, 1995, Vol. 23, No. 5 819-826
© 1995
MOLECULAR BIOLOGY |
Selectivity of the Escherichia coli RNA polymerase E
38 for overlapping promoters and ability to support CRP activation
Unité de Physicochimie des Macromolecules Biologiques URA 1149 du CNRS, 75724 Paris Cedex 15, France 1Department of Molecular Genetics, National Institute of Genetics Mishima, Shizuoka-ken 411, Japan
*To whom correspondence should be addressed
Received November 2, 1994. Revised January 20, 1995. Accepted January 20, 1995.
A series of gal promoter mutants has been used to compare the in vitro selectivities of the two forms of Escherichia coliRNA polymerase, E
38 and E70. In the absence of the CRP-cAMP complex, E38 shows a strong preference for the galP1 promoter, whereas E70 preferentially initiates transcription from the galP2 promoter. E38 selectivity is not affected by the nature and position of the upstream sequences or by the phasing between synthetic upstream curved sequences and the –10 regions. In fact, all effects of mutations in the extended –10 region can be accounted for without evoking strong new sequence preferences for E38. Finally, both E38 and E70 initiate transcription from the galP1 promoter in the presence of CRP-cAMP complex and support direct cAMP-CRP activation at several CRP-dependent promoters.
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