Nucleic Acids Research, 1995, Vol. 23, No. 5 861-868
© 1995
RNA |
The first and third uORFs in RSV leader RNA are efficiently translated: implications for translational regulation and viral RNA packaging
Department of Molecular Biology, University of Geneva 30 Quai E. Ansermet, 1211 Geneva 4, Switzerland
*To whom correspondence should be addressed at present address: Department of Biochemistry, McGill University, 3655 Drummond Street, Montréal, Quebec H3G 1Y6, Canada
Received September 1, 1994. Revised January 24, 1995. Accepted January 24, 1995.
Rous sarcoma virus (RSV) RNA leader contains three short upstream open reading frames. We have shown recently that bothuORFs 1 and 3 Influence in vivo translation of the downstream gag gene and are involved in the virus RNA packaging process. In this report, we have studied the translational events occurlng at the upstream AUGsin vivo. We show that (i) the first and third AUGs are efficient translational initiation sites; (ii) ribosomes reinitiate efficiently at AUG3; and (iii) deletions in the intercistronlc distance between uORF1 and 3 (which is well conserved among avian strains) prevent ribosome Initiation at AUG3, thus Increasing translation efficiency at the downstream AUGgag. The roles of the uORFs in translation and packaging are discussed.
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