Skip Navigation

This Article
Right arrow Print PDF (3284K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (12)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Sindelar, L. E.
Right arrow Articles by Jaklevic, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sindelar, L. E.
Right arrow Articles by Jaklevic, J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 6 982-987
© 1995

CHEMISTRY

High-throughput DNA synthesis in a multichannel format

Linda E. Sindelar* and Joe M. Jaklevic

Engineering Division, Lawrence Berkeley Laboratory, University of California Berkeley, CA 94720, USA

*To whom correspondence should be addressed

Received November 21, 1994. Revised January 24, 1995. Accepted January 24, 1995.

We describe an approach to high-throughput parallel DNA synthesis in which a multiwell format is used. The reactions are carried out in open wells using an argon ambient atmosphere to prevent reagent contamination. The controlled-pore glass beads which form the substrate for synthesis are held in individual wells with high-density polyethylene filter bottoms through which reagents are drawn into a vacuum manifold. The synthesis is carried out using direct reagent dispensing into the individual reaction wells. A computer controls the sequence in which reagents are dispensed and the timing of the periodic vacuum pulses required to synthesize the desired sequence. Experiments to date have demonstrated the viability of the approach for a variety of test sequences. Results obtained with HPLC analysis demonstrate coupling efficiencies as high as99.5% under optimized conditions. Use of the oligomers for DNA sequencing templates and as PCR primers has been demonstrated in production applications. The current instrument design consists of a series of discrete reaction chambers in a 12 channel module which can be multiplexed in a 12 x n format where n can be 1–8, i.e. 96 wells. A projected time interval for 12 parallel syntheses is 2.5 h, with 96 syntheses in 3.5 h. Becauseof the reduced volume of reagents required in the open well format, significant cost savings are projected.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
R. T. Pon and S. Yu
Tandem oligonucleotide synthesis using linker phosphoramidites
Nucleic Acids Res., April 6, 2005; 33(6): 1940 - 1948.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J.-Y. Cheng, H.-H. Chen, Y.-S. Kao, W.-C. Kao, and K. Peck
High throughput parallel synthesis of oligonucleotides with 1536 channel synthesizer
Nucleic Acids Res., September 15, 2002; 30(18): e93 - e93.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
S. Rayner, S. Brignac, R. Bumeister, Y. Belosludtsev, T. Ward, O'd. Grant, K. O'Brien, G. A. Evans, and H. R. Garner
MerMade: An Oligodeoxyribonucleotide Synthesizer for High Throughput Oligonucleotide Production in Dual 96-Well Plates
Genome Res., July 1, 1998; 8(7): 741 - 747.
[Abstract] [Full Text]

Home page
 Genome ResHome page
W Kimmerly, K Stultz, S Lewis, K Lewis, V Lustre, R Romero, J Benke, D Sun, G Shirley, C Martin, et al.
A P1-based physical map of the Drosophila euchromatic genome.
Genome Res., May 1, 1996; 6(5): 414 - 430.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.