Nucleic Acids Research, 1995, Vol. 23, No. 6 995-1002
© 1995
MOLECULAR BIOLOGY |
Protein binding interactions at the STB locus of the yeast 2µm plasmid
1Leicester Biocentre, University of Leicester Leicester LE1 7RH, UK 2Department of Genetics, University of Leicester Leicester LE1 7RH, UK
*To whom correspondence should be addressed at: Department of Genetics, University of Leicester, Leicester LE1 7RH, UK
Received November 14, 1994. Revised January 24, 1995. Accepted January 24, 1995.
The cis-acting STB locus has been shown to be a multiple protein binding site. STB-specific binding activity was detected in a normally insoluble yeast cell protein fraction, suggesting association with a sub-cellular structure. Both 2pm-encoded and host-encoded STB-binding activities were identified. The 2µm proteins showed contrasting STB-binding activities: C (REP2) protein acted cooperatively with the host factor to promote STB binding; B (REP1) protein also acted in association with the host factor, but showed a dual action, opposing or facilitating binding, depending upon concentration; D (RAF) exhibited rapid binding and antagonism to host factor binding. FLP did not bind, but promoted host factor dissociation. The implications of these activities for the molecular mechanism of 2µm plasmid inheritance are considered.
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