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Nucleic Acids Research, 1995, Vol. 23, No. 7 1140-1145
© 1995

MOLECULAR BIOLOGY

Drosophila immunity. A sequence homologous to mammalian interferon consensus response element enhances the activity of the diptericin promoter

Philippe Georgel*, Christine Kappler, Emma Langley, Isabelle Gross, Emmanuelle Nicolas, Jean-Marc Reichhart and Jules A. Hoffmann

Institut de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique 15, Rue René Descartes, 67084 Strasbourg Cedex, France

*To whom correspondence should be addressed

Received January 19, 1995. Revised February 27, 1995. Accepted February 27, 1995.

Bacterial challenge of larvae or adults of Drosophila induces the rapid transcription of several genes encoding antibacterial peptides with a large spectrum of activity. One of these peptides, the 82-residue anti-gram negative diptericin, is encoded by a single intronless gene and we are investigating the control of expression of this gene. Previous studies using both transgenic experiments and footprint analysis have highlighted the role in the induction of this gene of a 30 nucleotide region which contains three partially over lapping motifs with sequence homology to mammalian NF-kB and NF-IL6 response elements and to the GAAANN sequence present in the interferon consensus response elementsof some mammalian interferon-induced genes. We now show that the latter sequence binds in immune responsive tissues (fat body, blood cells) of Drosophila a -45 kDa polypeptide which cross-reacts with a polyserum directed against mammalian interferon Regulatory Factor-I. Using a transfection assay of Drosophila tumorous blood cells, we show that the GAAANN sequence positively regulates the activity of the diptericin promoter. We propose that this motif cooperatively interacts with the other response elements in the regulation of the diptericin gene expression.


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