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Nucleic Acids Research, 1995, Vol. 23, No. 7 1244-1251
© 1995

MOLECULAR BIOLOGY

Silkworm TFIIIA requires additional class III factors for commitment to transcription complex assembly on a 5S RNA gene

Timothy P. L. Smith1,3,+, Lisa S. Young1, Laurel B. Bender1,§ and Karen U. Sprague1,2,*

1Institute of Molecular Biology, University of Oregon Eugene, OR 97403, USA 2Department of Biology, University of Oregon Eugene, OR 97403, USA 3Department of Chemistry, University of Oregon Eugene, OR 97403, USA

*To whom correspondence should be addressed

Received October 13, 1994. Revised January 25, 1995. Accepted January 25, 1995.

We find striking similarities In promoter structure and requirements for template commitment on 5S RNA and tRNA genes from silkworms. The promoters are nearly the same size (~160 bp) and include flanking as well as internal sequences.To analyze the factor requirements for 5S RNA transcription complex assembly in a completely homologous system, we have isolated a silkworm fraction that is highly enriched for the 5S RNA-specific transcription factor, TFIIIA. Using this fraction, together with the other silkworm fractions, TFIIIB, TFIIIC, TFIIID and RNA polymerase III, we demonstrate that the requirements for 5S RNA transcription complex assembly are very similar to those previously established for a tRNACAla gene. Specifically, no individual factor fraction is sufficient for commitment of silkworm 5S RNA genes totranscription complex assembly. Rather, combinations of at least three factor fractions are required. Our observation thatmore than one subset of factors is competent for commitment suggests that silkworm 5S RNA genes further resemble tRNAcAla genes in their ability to use multiple pathways for transcription complex formation.

Present address: +Roman L. Hruska US Meat Animal Research Center, PO Box 166, Clay Center, NE 65933, USA

Present address: §Department of Biology, Indiana University, Bloomington, IN 47405, USA


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[Abstract] [PDF]


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