Skip Navigation

This Article
Right arrow Print PDF (735K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (40)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Chary, P.
Right arrow Articles by Lioyd, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chary, P.
Right arrow Articles by Lioyd, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 8 1398-1405
© 1995

ENZYMOLOGY

In vitro replication by prokaryotic and eukaryotic polymerases on DNA templates containing site-specific and stereospecific benzo [a]pyrene-7, 8-dihydrodiol-9, 10-epoxide adducts

Parvathi Chary and Stephen Lioyd*

Sealy Center for Molecular Science, The University of Texas Medical Branch Galveston, TX 77555, USA

*To whom correspondence should be addressed

Received December 1, 1994. Revised March 9, 1995. Accepted March 9, 1995.

DNA adducts of the environmental carcinogen benzo[a]pyrene-7, 8-dihydrodiol-9, 10-epoxide (BPDE) interact stereospecifically with prokaryotic and eukaryotic polymerases in vitro. Toward understanding the capacity to replicate past different diastereomers of BPDE at specific sites in DNA, six deoxyoligonucleotides, each 33 baseslong, were constructed with stereochemically defined BPDE adducts on adenine N6 at position two of the human N-ras codon 61. Four polymerases that were studied under single en counters with the template-primer complex terminated synthesis one base 3' to the lesion with all the adducted templates. When multiple encounters between polymerase and substrate were permitted, each of the polymerases analyzed revealed a unique pattern for a given adducted template. The general replication pattern was encompassed under two categories, reflecting the significance of the A and S configurations of C10 of the pyrenyl ring attached to the single-stranded DNA template. Furthermore, within each of these categories, every polymerase demon strated distinct quantitative differences in product accumulation at a given site, for the various adducted templates. Among the polymerases utilized in this study, exonuclease-deficient Klenow fragment of polymerase I (exo–KF) exhibited the most efficient translesion synthesis resulting in ~1 6% full-length products with the modified templates bearing adducts with C10–S configuration. In contrast, chain elongatlon with bacteriophage T4 DNA polymerase bearing an active 3'->5' exonucleolytic activity was most strongly inhibited by all six BPDE-adducted templates. Misincorporation of A opposite the adduct occurred in all the templates when polymerized with Sequenase, whereas exo– KF preferentially incorporated C opposite the C10–R BPDE adducts and A opposite the C10– BPDE adducts.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
O. Rechkoblit, Y. Zhang, D. Guo, Z. Wang, S. Amin, J. Krzeminsky, N. Louneva, and N. E. Geacintov
trans-Lesion Synthesis Past Bulky Benzo[a]pyrene Diol Epoxide N2-dG and N6-dA Lesions Catalyzed by DNA Bypass Polymerases
J. Biol. Chem., August 16, 2002; 277(34): 30488 - 30494.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Shen, J. M. Sayer, H. Kroth, I. Ponten, M. O'Donnell, R. Woodgate, D. M. Jerina, and M. F. Goodman
Efficiency and Accuracy of SOS-induced DNA Polymerases Replicating Benzo[a]pyrene-7,8-diol 9,10-Epoxide A and G Adducts
J. Biol. Chem., February 8, 2002; 277(7): 5265 - 5274.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
H. Zhang, G. Marra, J. Jiricny, V. M. Maher, and J.J. McCormick
Mismatch repair is required for O6-methylguanine-induced homologous recombination in human fibroblasts
Carcinogenesis, September 1, 2000; 21(9): 1639 - 1646.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. Remington, S. E. Bennett, C. M. Harris, T. M. Harris, and K. Bebenek
Highly Mutagenic Bypass Synthesis by T7 RNA Polymerase of Site-specific Benzo[a]pyrene Diol Epoxide-adducted Template DNA
J. Biol. Chem., May 22, 1998; 273(21): 13170 - 13176.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. F. Hashim, N. Schnetz-Boutaud, and L. J. Marnett
Replication of Template-Primers Containing Propanodeoxyguanosine by DNA Polymerase beta . INDUCTION OF BASE PAIR SUBSTITUTION AND FRAMESHIFT MUTATIONS BY TEMPLATE SLIPPAGE AND DEOXYNUCLEOSIDE TRIPHOSPHATE STABILIZATION
J. Biol. Chem., August 8, 1997; 272(32): 20205 - 20212.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Chary, C. M. Harris, T. M. Harris, and R. S. Lloyd
Differential Tolerance to DNA Polymerization by HIV-1 Reverse Transcriptase on N6 Adenine C10R and C10S Benzo[a]pyrene-7,8-dihydrodiol 9,10-Epoxide-adducted Templates
J. Biol. Chem., February 28, 1997; 272(9): 5805 - 5813.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.