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Nucleic Acids Research, 1995, Vol. 23, No. 8 1419-1425
© 1995

MOLECULAR BIOLOGY

Increased frequency of deletions in the mitochondrial genome with age of Caenorhabditis elegans

S. Melov1,*, G. J. Lithgow1, D. R. Fischer1, P. M. Tedesco1 and T. E. Johnson1,2

1Institute for Behavioral Genetics, University of Colorado Boulder, CO 80309, USA 2Department of Psychology, University of Colorado Boulder, CO 80309, USA

*To whom correspondence should be addressed at present address: Department of Molecular Genetics and Medicine, School of Medicine, Emory University, 1462 Clifton Road, NE Atlanta, GA 30322, USA

Received November 18, 1994. Revised March 3, 1995. Accepted March 3, 1995.

We have developed a long-extension-PCR strategy which amplifies approximately half of the mitochondrial genome (6.3 kb)of Caenorhabditis elegans using an individual worm as target. We analyzed three strains over their life span to assess the number of detectable deletions in the mitochondrial genome. Two of these strains are wild-type for life span while the third is mutant in the age-1 gene, approximately doubling its maximum life span. At the mean life span in wild-type strains, there was a significant difference between the frequency of deletions detected in the mitochondrial genome compared with the mean number of deletions in young animals. In addition, deletions in the mitochondrial genome occur at a significantly lower rate in age-1 mutants as compared with wild type. We cloned and identified the breakpoints of two deletions and found that one of the deletions had a direct repeat of 8 bp at the breakpoint. This is the largest single study (over 900 individual animals) characterizing the frequency of deletions in the mitochondrial genome as a function of age yet carried out.


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