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Nucleic Acids Research, 1995, Vol. 23, No. 9 1468-1474
© 1995

MOLECULAR BIOLOGY

The lytic replicon of bacteriophage P1 is controlled by an antisense RNA

Jochen Heinrich, Hans-Dieter Riedel+, Beate Rückert, Rudi Lurz and Heinz Schuster*

Max-Planck-lnstitut für Molekulare Genetik D-14195 Berlin, Germany

*To whom correspondence should be addressed

Received February 14, 1995. Accepted March 14, 1995.

The lytic replicon of phage P1 is used for DNA replication during the lytic cycle. It comprises about 2% of the P1 genome and contains the P1 C1 repressor-cont rolled operator-promoter element Op53.P53 and the kllA and the repL genes, in that order. Transcription of the lytic replicon of P53 and synthesis of the product of repL, but not kllA, are required for replicon function. We have identified an additional promoter, termed P53as (antisense), at the 5'-end of the KllA gene from which a 180 base transcript Is constitutively synthesized and in the opposite direction to the P53 transcript. By using a promoter probe plasmld we show that transcription from P53 is strongly repressed by the C1 repressor, whereas that of P53as remains unaffected. Accordingly, the C1 repressor Inhibits binding of Escherichia coll RNA polymerase to P53, but not to P53as, as shown by electron microscopy. Under non-repressed conditions transcription from P53 appears to be inhibited by P53as activity and vice versa. An inhibitory effect of P53as on the P1 lytic replicon was revealed by the construction and characterization of a Pittas promoter-down mutant. Under non-repressed conditions transcription of repL and, as a consequence, replication of the plasmld Is strongly enhanced when Pittas is Inactive. The results suggest a regulatory role for Pittas on the P1 lytic replicon.

+Present address: Universitätsklinik Heidelberg, D-69115 Heidelberg, Germany


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