Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (145K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Wei, T
Right arrow Articles by Bernander, R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wei, T
Right arrow Articles by Bernander, R
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 24, Issue 10 1865-1872, Copyright © 1996 by Oxford University Press

ARTICLES

Interaction of the IciA protein with AT-rich regions in plasmid replication origins

T Wei and R Bernander
Department of Microbiology, Biomedical Center, Uppsala University, Sweden.

A set of AT-rich repeats is a common motif in prokaryotic replication origins. We have screened for proteins binding to the AT-rich repeat region in plasmids F, R1 and pSC101 using an electrophoretic mobility shift assay with PCR-amplified DNA fragments from the origins. The IciA protein, which is known to bind to the AT-rich repeat region in the Escherichia coli origin of chromosome replication, oriC, was found to bind to the corresponding region from plasmids F (oriS) and R1, but not to pSC101. DNase I footprint analysis showed that IciA interacted with the AT-rich region in both F and R1. When the IciA gene was deleted, the copy number of plasmid F increased somewhat, whereas there was no major effect on the replication of pSC101 and R1, or on the E. coli chromosome.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
R. S. Laishram and J. Gowrishankar
Environmental regulation operating at the promoter clearance step of bacterial transcription
Genes & Dev., May 15, 2007; 21(10): 1258 - 1272.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. A. Azam and A. Ishihama
Twelve Species of the Nucleoid-associated Protein from Escherichia coli. SEQUENCE RECOGNITION SPECIFICITY AND DNA BINDING AFFINITY
J. Biol. Chem., November 12, 1999; 274(46): 33105 - 33113.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.