Nucleic Acids Research, Vol 24, Issue 10 1873-1878, Copyright © 1996 by Oxford University Press
Y Makino, T Ohga, S Toh, K Koike, K Okumura, M Wada, M Kuwano and K Kohno
We have isolated three overlapping genomic clones containing the 5'portion
of the human YB-1 gene. These clones span approximately 25 kb of contiguous
DNA containing 10 kb of 5' flanking sequence and 15 kb of the gene. The
nucleotide sequence of the first exon and of 2000 upstream base pairs (bp)
was determined. The first axon is unusually large and contains a 166 bp
coding sequence and a 331 bp untranslated region. CpG sequences cover the
5'-end of the YB-1 gene including its first axon and intron as well as the
upstream regions. The GC content around the first exon is approximately 70%
and a CpG-free region was located in the untranslated sequence. The segment
preceding the major transcription initiation site does not contain a TATA
box, CCAAT box and the binding sequence for known transcription factors. A
transient expression assay using the chloramphenicol acetyltransferase
(CAT) gene showed that the sequence from +24 to +281 was critical for CAT
expression. Fluorescence in situ hybridization demonstrated the chromosomal
locus of YB-1 gene on chromosome 1p34. Polymerase chain reaction analysis
on other genomic phage DNAs showed that several clones were derived from
pseudogenes.
ARTICLES
Structural and functional analysis of the human Y-box binding protein (YB-1) gene promoter
Department of Biochemistry, Kyushu University School of Medicine, Japan.
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