Nucleic Acids Research, Vol 24, Issue 10 1913-1920, Copyright © 1996 by Oxford University Press
DB Schubart, P Sauter, S Massa, EM Friedl, H Schwarzenbach and P Matthias
The B cell-specific activity of immunoglobulin (Ig) gene promoters is to a
large extent mediated by the conserved octamer motif ATTTGCAT. This
requires the DNA binding octamer factors Oct-1 and/or Oct-2, as well as an
additional B cell-restricted non-DNA binding cofactor. We recently cloned
such a coactivator specific for Oct-1 or Oct-2 from human B cells and
called it OBF-1. Here we report the isolation and characterization of the
murine homologue. Full-length cDNA clones as well as genomic clones were
isolated and the gene structure was determined. The deduced protein
sequence shows that the mouse protein has an identical length, is likewise
proline rich and shows 89% overall identity to the human protein. The OBF-1
gene is expressed in a very highly B cell-specific manner and is
transcribed in cells representative of all stages of B cell
differentiation, including the earliest ones. We show that OBF-1 interacts
in the absence of DNA with the POU domain of Oct-1 or Oct-2 and also with
the general transcription factors TBP and TFIIB. Furthermore, we
demonstrate that although OBF-1 efficiently activates promoter octamer
sites, it does not activate enhancer octamer sites.
ARTICLES
Gene structure and characterization of the murine homologue of the B cell-specific transcriptional coactivator OBF-1 [published erratum appears in Nucleic Acids Res 1996 Jun 15;24(12):2462]
Friedrich Miescher Institute, Basel, Switzerland.
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