Nucleic Acids Research, Vol 24, Issue 10 1943-1949, Copyright © 1996 by Oxford University Press
J Li and RE Pearlman
During macronuclear development in the ciliate Tetrahymena thermophila,
extensive rearrangement events occur as DNA deletions. We have studied a
developmentally programmed deletion called mse2.9 that occurs within an
intron in a gene in both genomic DNA and in an rDNA vector introduced into
the cell by transformation. Extensive microheterogeneity at the deletion
junctions has been found in caryonidal strains and in the rDNA in
transformed cells. A transformation assay has been used to identify
sequences required for proper processing of mse2.9. Models to explain
deletion site selection as well as microheterogeneity at junction sites are
presented.
ARTICLES
Programmed DNA rearrangement from an intron during nuclear development in Tetrahymena thermophila: molecular analysis and identification of potential cis-acting sequences
Department of Biology, York University, Toronto, Ontario, Canada.
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