Nucleic Acids Research, Vol 24, Issue 11 2005-2010, Copyright © 1996 by Oxford University Press
T Yasugi and PM Howley
Ubiquitin conjugating enzymes (UBCs) are a family of proteins directly
involved in ubiquitination of proteins. Ubiquitination is known to be
involved in control of a variety of cellular processes, including cell
proliferation, through the targeting of key regulatory proteins for
degradation. The ubc9 gene of the yeast Saccharomyces cerevisiae (Scubc9)
is an essential gene which is required for cell cycle progression and is
involved in the degradation of S phase and M phase cyclins. We have
identified a human homolog of Scubc9 (termed hubc9) using the two hybrid
screen for proteins that interact with the human papillomavirus type 16 E1
replication protein. The hubc9 encoded protein shares a very high degree of
amino acid sequence similarity with ScUBC9 and with the homologous hus5+
gene product of Schizosaccharomyces pombe. Genetic complementation
experiments in a S.cerevisiae ubc9ts mutant reveal that hUBC9 can
substitute for the function of ScUBC9 required for cell cycle progression.
ARTICLES
Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9
Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Tomoiu, A. Gravel, R. M. Tanguay, and L. Flamand Functional Interaction between Human Herpesvirus 6 Immediate-Early 2 Protein and Ubiquitin-Conjugating Enzyme 9 in the Absence of Sumoylation. J. Virol., October 1, 2006; 80(20): 10218 - 10228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. A. M. van Waardenburg, D. M. Duda, C. S. Lancaster, B. A. Schulman, and M.-A. Bjornsti Distinct functional domains of ubc9 dictate cell survival and resistance to genotoxic stress. Mol. Cell. Biol., July 1, 2006; 26(13): 4958 - 4969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Castillo, L. J. Kong, L. Hanley-Bowdoin, and E. R. Bejarano Interaction between a Geminivirus Replication Protein and the Plant Sumoylation System J. Virol., March 15, 2004; 78(6): 2758 - 2769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hofmann, S. Flöss, and T. Stamminger Covalent Modification of the Transactivator Protein IE2-p86 of Human Cytomegalovirus by Conjugation to the Ubiquitin-Homologous Proteins SUMO-1 and hSMT3b J. Virol., March 15, 2000; 74(6): 2510 - 2524. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Trost, G. Kochs, and O. Haller Characterization of a Novel Serine/Threonine Kinase Associated with Nuclear Bodies J. Biol. Chem., March 15, 2000; 275(10): 7373 - 7377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Liu, C. Jin, X. Liao, Z. Shen, D. J. Chen, and Y. Chen The Binding Interface between an E2 (UBC9) and a Ubiquitin Homologue (UBL1) J. Biol. Chem., June 11, 1999; 274(24): 16979 - 16987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Koldamova, I. M. Lefterov, M. T. DiSabella, and J. S. Lazo An Evolutionarily Conserved Cysteine Protease, Human Bleomycin Hydrolase, Binds to the Human Homologue of Ubiquitin-Conjugating Enzyme 9 Mol. Pharmacol., December 1, 1998; 54(6): 954 - 961. [Abstract] [Full Text]
|
|
|
|
|
|
G. W. Lee, F. Melchior, M. J. Matunis, R. Mahajan, Q. Tian, and P. Anderson Modification of Ran GTPase-activating Protein by the Small Ubiquitin-related Modifier SUMO-1 Requires Ubc9, an E2-type Ubiquitin-conjugating Enzyme Homologue J. Biol. Chem., March 13, 1998; 273(11): 6503 - 6507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Firestein and N. Feuerstein Association of Activating Transcription Factor 2 (ATF2) with the Ubiquitin-conjugating Enzyme hUBC9. IMPLICATION OF THE UBIQUITIN/PROTEASOME PATHWAY IN REGULATION OF ATF2 IN T CELLS J. Biol. Chem., March 6, 1998; 273(10): 5892 - 5902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Schwarz, K. Matuschewski, D. Liakopoulos, M. Scheffner, and S. Jentsch The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme PNAS, January 20, 1998; 95(2): 560 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Sternsdorf, K. Jensen, and H. Will Evidence for Covalent Modification of the Nuclear Dot-associated Proteins PML and Sp100 by PIC1/SUMO-1 J. Cell Biol., December 29, 1997; 139(7): 1621 - 1634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gong, T. Kamitani, K. Fujise, L. S. Caskey, and E. T. H. Yeh Preferential Interaction of Sentrin with a Ubiquitin-conjugating Enzyme, Ubc9 J. Biol. Chem., November 7, 1997; 272(45): 28198 - 28201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tong, G. Hateboer, A. Perrakis, R. Bernards, and T. K. Sixma Crystal Structure of Murine/Human Ubc9 Provides Insight into the Variability of the Ubiquitin-conjugating System J. Biol. Chem., August 22, 1997; 272(34): 21381 - 21387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kumar, W. H. Kao, and P. M. Howley Physical Interaction between Specific E2 and Hect E3 Enzymes Determines Functional Cooperativity J. Biol. Chem., May 23, 1997; 272(21): 13548 - 13554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Saitoh, R. Pu, M. Cavenagh, and M. Dasso RanBP2 associates with Ubc9p and a modified form of RanGAP1 PNAS, April 15, 1997; 94(8): 3736 - 3741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-J. Kho, G. S. Huggins, W. O. Endege, C.-M. Hsieh, M.-E. Lee, and E. Haber Degradation of E2A Proteins through a Ubiquitin-conjugating Enzyme, UbcE2A J. Biol. Chem., February 7, 1997; 272(6): 3845 - 3851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Long and L. C. Griffith Identification and Characterization of a SUMO-1 Conjugation System That Modifies Neuronal Calcium/Calmodulin-dependent Protein Kinase II in Drosophila melanogaster J. Biol. Chem., December 22, 2000; 275(52): 40765 - 40776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Ivanova, S. V. Ivanov, A. Danilkovitch-Miagkova, and M. I. Lerman Regulation of STRA13 by the von Hippel-Lindau Tumor Suppressor Protein, Hypoxia, and the UBC9/Ubiquitin Proteasome Degradation Pathway J. Biol. Chem., April 27, 2001; 276(18): 15306 - 15315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Kurtzman and N. Schechter Ubc9 interacts with a nuclear localization signal and mediates nuclear localization of the paired-like homeobox protein Vsx-1 independent of SUMO-1 modification PNAS, May 8, 2001; 98(10): 5602 - 5607. [Abstract] [Full Text] [PDF]
|
|