Nucleic Acids Research, Vol 24, Issue 11 2011-2016, Copyright © 1996 by Oxford University Press
R Abe, K Yamamoto and H Sakamoto
We have identified cDNAs encoding Mel-N1, the mouse homologue of a human
nervous system-specific RNA-binding protein, Hel-N1. Two major mRNA
transcripts of Mel-N1 were detected predominantly in the adult mouse brain
by Northern blot analysis. To gain insight into the RNA binding specificity
of Mel-N1, we performed iterative in vitro RNA selection. The resulting in
vitro selected RNAs were found to contain AU-rich sequences as well as a
GAAA motif in the majority of clones. By means of in vitro binding assays
we demonstrate that this GAAA sequence appears to significantly affect the
Mel-N1 RNA-binding efficiency. Our studies further reveal that Mel-N1 can
bind to its own 3' untranslated region (3'UTR) as well as to the c-fos
3'UTR, and is localized predominantly in the cytoplasmic region in cells,
suggesting that posttranscriptional autoregulation of Mel-N1 gene
expression occurs in vivo.
ARTICLES
Target specificity of neuronal RNA-binding protein, Mel-N1: direct binding to the 3' untranslated region of its own mRNA
Department of Biology, Faculty of Science, Kobe University, Japan.
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