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Nucleic Acids Research, Vol 24, Issue 11 2017-2021, Copyright © 1996 by Oxford University Press

ARTICLES

The exon sequence TAGG can inhibit splicing

F Del Gatto, MC Gesnel and R Breathnach
INSERM U211, Institut de Biologie, Nantes, France.

The fibroblast growth factor receptor-2 gene contains a pair of alternative exons, K-SAM and BEK, which are spliced in a cell type specific manner. We have shown previously that a 10 nucleotide sequence within the K-SAM exon exerts a negative effect on K-SAM exon splicing independent of cell type. We demonstrate here that this sequence works autonomously, as it can repress splicing of a heterologous exon, the EIIIb alternative exon of the rat fibronectin gene. By introducing point mutations into the 10 nucleotide sequence, we have shown that the functional portion is limited to 4 nucleotides, TAGG, the dinucleotide AG of which is particularly important. This short sequence may participate in the control of splicing of exons carrying it, provided that they carry weak splice sites.
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