Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (113K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Morrison, C
Right arrow Articles by Wagner, E
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morrison, C
Right arrow Articles by Wagner, E
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 24, Issue 11 2053-2058, Copyright © 1996 by Oxford University Press

ARTICLES

Extrachromosomal recombination occurs efficiently in cells defective in various DNA repair systems

C Morrison and E Wagner
Research Institute of Molecular Pathology, Vienna, Austria.

A series of different frameshift mutations of a firefly luciferase reporter plasmid was created so that no activity was obtained when they were transfected into mammalian cells. Co-transfection of these constructs with short fragments of the original sequence resulted in luciferase activity in different cell lines (A-549, NIH 3T3 and Jurkat). The level of this activity was dependent on the length of the fragment, regardless of cell line examined. Two different transfection techniques (lipofection and adenovirus-enhanced gene transfer) gave similar results. It was shown by polymerase chain reaction that expression of detectable luciferase required recombination of the transfected molecules. Cells with defined defects in DNA repair pathways were examined for their ability to perform this extrachromosomal recombination. Cells lacking normal Ku p80, (ADP- ribosyl)transferase, MLH1 or XP-C were all capable of restoring expression to the frameshifted constructs. Given the pivotal roles of the above molecules in the pathways of DNA repair, it seems that this recombination derives from a different activity.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
P. Manivasakam, J. Aubrecht, S. Sidhom, and R. H. Schiestl
Restriction enzymes increase efficiencies of illegitimate DNA integration but decrease homologous integration in mammalian cells
Nucleic Acids Res., December 1, 2001; 29(23): 4826 - 4833.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. M.-d. Murcia, M. Mark, O. Wendling, A. Wynshaw-Boris, and G. de Murcia
Early Embryonic Lethality in PARP-1 Atm Double-Mutant Mice Suggests a Functional Synergy in Cell Proliferation during Development
Mol. Cell. Biol., March 1, 2001; 21(5): 1828 - 1832.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. E. Masson and J. Paszkowski
Arabidopsis thaliana mutants altered in homologous recombination
PNAS, October 14, 1997; 94(21): 11731 - 11735.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Z.-Q. Wang, L. Stingl, C. Morrison, M. Jantsch, M. Los, K. Schulze-Osthoff, and E. F. Wagner
PARP is important for genomic stability but dispensable in apoptosis
Genes & Dev., September 15, 1997; 11(18): 2347 - 2358.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.