Nucleic Acids Research, Vol 24, Issue 11 2067-2072, Copyright © 1996 by Oxford University Press
Y Tsukamoto, J Kato and H Ikeda
Hdf1 is the yeast homologue of the mammalian 70 kDa subunit of Ku- protein,
which has DNA end-binding activity and is involved in DNA double-strand
break repair and V(D)J recombination. To examine whether Hdf1 is involved
in illegitimate recombination, we have measured the rate of deletion
mutation caused by illegitimate recombination on a plasmid in an hdf1
disruptant. The hdf1 mutation reduced the rate of deletion formation by
20-fold, while it did not affect mitotic and meiotic homologous
recombinations between two heteroalleles or homologous recombination
between direct repeats. Hence Hdf1 participates in illegitimate
recombination, but not in homologous recombination, in contrast to Rad52,
Rad50, Mre11 and Xrs2, which are involved in both homologous and
illegitimate recombination. The illegitimate recombination in the hdf1
disruptant took place between recombination sites that shared short regions
of homology (1-4 bp), as was observed in the wild-type. Based on the DNA
end-binding activity of Hdf1, we discuss models in which Hdf1 plays an
important role in the late step of illegitimate recombination.
ARTICLES
Hdf1, a yeast Ku-protein homologue, is involved in illegitimate recombination, but not in homologous recombination
Department of Molecular Biology, Institute of Medical Science, University of Tokyo, Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Tomita, A. Matsuura, T. Caspari, A. M. Carr, Y. Akamatsu, H. Iwasaki, K.-i. Mizuno, K. Ohta, M. Uritani, T. Ushimaru, et al. Competition between the Rad50 Complex and the Ku Heterodimer Reveals a Role for Exo1 in Processing Double-Strand Breaks but Not Telomeres Mol. Cell. Biol., August 1, 2003; 23(15): 5186 - 5197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sabourin, J. L. Nitiss, K. C. Nitiss, K. Tatebayashi, H. Ikeda, and N. Osheroff Yeast recombination pathways triggered by topoisomerase II-mediated DNA breaks Nucleic Acids Res., August 1, 2003; 31(15): 4373 - 4384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Manthey and A. M. Bailis Multiple Pathways Promote Short-Sequence Recombination in Saccharomyces cerevisiae Mol. Cell. Biol., August 1, 2002; 22(15): 5347 - 5356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Frank-Vaillant and S. Marcand NHEJ regulation by mating type is exercised through a novel protein, Lif2p, essential to the Ligase IV pathway Genes & Dev., November 15, 2001; 15(22): 3005 - 3012. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Pluth, L. M. Fried, and C. U. Kirchgessner Severe Combined Immunodeficient Cells Expressing Mutant hRAD54 Exhibit a Marked DNA Double-Strand Break Repair and Error-prone Chromosome Repair Defect Cancer Res., March 1, 2001; 61(6): 2649 - 2655. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. A. Clikeman, G. J. Khalsa, S. L. Barton, and J. A. Nickoloff Homologous Recombinational Repair of Double-Strand Breaks in Yeast Is Enhanced by MAT Heterozygosity Through yKU-Dependent and -Independent Mechanisms Genetics, February 1, 2001; 157(2): 579 - 589. [Abstract] [Full Text]
|
|
|
|
|
|
P. J. McHugh, W. R. Sones, and J. A. Hartley Repair of Intermediate Structures Produced at DNA Interstrand Cross-Links in Saccharomyces cerevisiae Mol. Cell. Biol., May 15, 2000; 20(10): 3425 - 3433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Fellerhoff, F. Eckardt-Schupp, and A. A. Friedl Subtelomeric Repeat Amplification Is Associated With Growth at Elevated Temperature in yku70 Mutants of Saccharomyces cerevisiae Genetics, March 1, 2000; 154(3): 1039 - 1051. [Abstract] [Full Text]
|
|
|
|
|
|
C. W. Moore, J. McKoy, M. Dardalhon, D. Davermann, M. Martinez, and D. Averbeck DNA Damage-Inducible and RAD52-Independent Repair of DNA Double-Strand Breaks in Saccharomyces cerevisiae Genetics, March 1, 2000; 154(3): 1085 - 1099. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. DE LANGE and J.H.J. PETRINI A New Connection at Human Telomeres: Association of the Mre11 Complex with TRF2 Cold Spring Harb Symp Quant Biol, January 1, 2000; 65(0): 265 - 274. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Bianchi and T. de Lange Ku Binds Telomeric DNA in Vitro J. Biol. Chem., July 23, 1999; 274(30): 21223 - 21227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. T. Paull and M. Gellert Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the Mre11/Rad50 complex Genes & Dev., May 15, 1999; 13(10): 1276 - 1288. [Abstract] [Full Text]
|
|
|
|
|
|
S. Le, J. K. Moore, J. E. Haber, and C. W. Greider RAD50 and RAD51 Define Two Pathways That Collaborate to Maintain Telomeres in the Absence of Telomerase Genetics, May 1, 1999; 152(1): 143 - 152. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Yamagata, J.-i. Kato, A. Shimamoto, M. Goto, Y. Furuichi, and H. Ikeda Bloom's and Werner's syndrome genes suppress hyperrecombination in yeast sgs1 mutant: Implication for genomic instability in human diseases PNAS, July 21, 1998; 95(15): 8733 - 8738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Connelly, L. A. Kirkham, and D. R. F. Leach The SbcCD nuclease of Escherichia coli is a structural maintenance of chromosomes (SMC) family protein that cleaves hairpin DNA PNAS, July 7, 1998; 95(14): 7969 - 7974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gravel, M. Larrivee, P. Labrecque, and R. J. Wellinger Yeast Ku as a Regulator of Chromosomal DNA End Structure Science, May 1, 1998; 280(5364): 741 - 744. [Abstract] [Full Text]
|
|
|
|
|
|
L. K. Lewis, J. M. Kirchner, and M. A. Resnick Requirement for End-Joining and Checkpoint Functions, but Not RAD52-Mediated Recombination, after EcoRI Endonuclease Cleavage of Saccharomyces cerevisiae DNA Mol. Cell. Biol., April 1, 1998; 18(4): 1891 - 1902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Manivasakam and R. H. Schiestl Nonhomologous End Joining during Restriction Enzyme-Mediated DNA Integration in Saccharomyces cerevisiae Mol. Cell. Biol., March 1, 1998; 18(3): 1736 - 1745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Friedl, M. Kiechle, B. Fellerhoff, and F. Eckardt-Schupp Radiation-Induced Chromosome Aberrations in Saccharomyces cerevisiae : Influence of DNA Repair Pathways Genetics, March 1, 1998; 148(3): 975 - 988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Schar, G. Herrmann, G. Daly, and T. Lindahl A newly identified DNA ligase of Saccharomyces cerevisiae involved in RAD52-independent repair of DNA double-strand breaks Genes & Dev., August 1, 1997; 11(15): 1912 - 1924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Feldmann, L. Driller, B. Meier, G. Mages, J. Kellermann, and E.-L. Winnacker HDF2, the Second Subunit of the Ku Homologue from Saccharomyces cerevisiae J. Biol. Chem., November 1, 1996; 271(44): 27765 - 27769. [Abstract] [Full Text] [PDF]
|
|