Nucleic Acids Research, Vol 24, Issue 11 2112-2118, Copyright © 1996 by Oxford University Press
MA Vigano and LM Staudt
Oct-3, a member of the POU family of transcription factors, is expressed in
pluripotent cells of early mammalian embryos and in undifferentiated
embryonal carcinoma cell lines. Using a variety of Oct- 3 mutants, we have
identified two different domains of Oct-3 which activate transcription in
transfected mammalian cells. One of these domains, located in the
C-terminal part of the protein, plays a major role in transcriptional
activation when Oct-3 is bound to its cognate site, the octamer motif. An
Oct-3 mutant containing a single amino acid substitution in the POU
homeodomain is unable to bind the octamer target in vitro, yet is still
able to activate transcription in an octamer-dependent manner. We provide
evidence that transactivation by this mutant involves protein-protein
interactions with the ubiquitous octamer binding factor Oct-1. This
interaction requires the POU- specific domain of Oct-3 and allows
recruitment of Oct-3 to the target promoter even in the absence of Oct-3
DNA binding.
ARTICLES
Transcriptional activation by Oct-3: evidence for a specific role of the POU-specific domain in mediating functional interaction with Oct-1
DIBIT-Istituto Scientifico H.S. Raffaele, Milano, Italy.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Lee, H. K. Kim, J.-Y. Rho, Y.-M. Han, and J. Kim The Human OCT-4 Isoforms Differ in Their Ability to Confer Self-renewal J. Biol. Chem., November 3, 2006; 281(44): 33554 - 33565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. F. Smith and K. G. Ford Use of altered-specificity binding Oct-4 suggests an absence of pluripotent cell-specific cofactor usage Nucleic Acids Res., October 20, 2005; 33(18): 6011 - 6023. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Andersen and M. G. Rosenfeld POU Domain Factors in the Neuroendocrine System: Lessons from Developmental Biology Provide Insights into Human Disease Endocr. Rev., February 1, 2001; 22(1): 2 - 35. [Abstract] [Full Text]
|
|
|
|
|
|
T. K. Nowling, L. R. Johnson, M. S. Wiebe, and A. Rizzino Identification of the Transactivation Domain of the Transcription Factor Sox-2 and an Associated Co-activator J. Biol. Chem., February 11, 2000; 275(6): 3810 - 3818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hildesheim, R. A. Foster, M. E. Chamberlin, and J. C. Vogel Characterization of the Regulatory Domains of the Human Skn-1a/Epoc-1/Oct-11 POU Transcription Factor J. Biol. Chem., September 10, 1999; 274(37): 26399 - 26406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Brehm, K. Ohbo, W. Zwerschke, V. Botquin, P. Jansen-Durr, and H. R. Scholer Synergism with Germ Line Transcription Factor Oct-4: Viral Oncoproteins Share the Ability To Mimic a Stem Cell-Specific Activity Mol. Cell. Biol., April 1, 1999; 19(4): 2635 - 2643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ben-Shushan, J. R. Thompson, L. J. Gudas, and Y. Bergman Rex-1, a Gene Encoding a Transcription Factor Expressed in the Early Embryo, Is Regulated via Oct-3/4 and Oct-6 Binding to an Octamer Site and a Novel Protein, Rox-1, Binding to an Adjacent Site Mol. Cell. Biol., April 1, 1998; 18(4): 1866 - 1878. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. A. Eraly, S. B. Nelson, K. M. Huang, and P. L. Mellon Oct-1 Binds Promoter Elements Required for Transcription of the GnRH Gene Mol. Endocrinol., April 1, 1998; 12(4): 469 - 481. [Abstract] [Full Text]
|
|
|
|
 |
|
 A K Ryan and M G Rosenfeld POU domain family values: flexibility, partnerships, and developmental codes. Genes & Dev., May 15, 1997; 11(10): 1207 - 1225. [PDF]
|
|
|
|
|
|
D.-C. Ambrosetti, H. R. Scholer, L. Dailey, and C. Basilico Modulation of the Activity of Multiple Transcriptional Activation Domains by the DNA Binding Domains Mediates the Synergistic Action of Sox2 and Oct-3 on the Fibroblast Growth Factor-4 Enhancer J. Biol. Chem., July 21, 2000; 275(30): 23387 - 23397. [Abstract] [Full Text] [PDF]
|
|