Nucleic Acids Research, Vol 24, Issue 12 2347-2351, Copyright © 1996 by Oxford University Press
AS Bournay, PE Hedley, A Maddison, R Waugh and GC Machray
We show that two invertase genes in potato, like most other plant invertase
genes, include a very short second exon of 9 bp which encodes the central
three amino acids of a motif highly conserved in invertases of diverse
origin. This mini-exon is one of the smallest known in plants and pre-mRNA
from these genes may be susceptible to alternative splicing, because of a
potential requirement for specialized interaction with the splicing
machinery to ensure correct processing for the production of a mature mRNA.
No evidence of aberrant post- transcriptional processing was observed
during normal invertase gene expression in potato. The fidelity of
post-transcriptional processing of the pre-mRNA from one of the genes was
perturbed by cold stress, resulting in the deletion of the mini-exon from
some transcripts. This alternative splicing event occurred under cold
stress in both leaf and stem, but was not induced by wounding. This adds an
example of exon skipping and the induction of alternative processing by
cold stress to the small number of transcripts which have been shown to
exhibit alternative splicing in plants. The differential sensitivity of
post- transcriptional processing to cold stress observed for the two
transcripts examined will permit further dissection of the nucleotide
sequence requirements for their accurate splicing.
ARTICLES
Exon skipping induced by cold stress in a potato invertase gene transcript
Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Invergowrie,Dundee, UK.
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