Nucleic Acids Research, Vol 24, Issue 12 2387-2394, Copyright © 1996 by Oxford University Press
V Mach, K Ohno, H Kokubo and Y Suzuki
The Drosophila Fork head protein participates in salivary gland formation,
since salivary glands are missing in fork head embryos. Here we show that
the fork head encoded protein binds to an upstream regulatory region of the
larval salivary gland glue protein gene Sgs3. Mobility shift assay in the
presence of an anti-Fork head antibody demonstrated that the Fork head
factor interacts with the TGTTTGC box shown to be involved in
tissue-specific Sgs3 expression. Experiments employing a set of
oligonucleotide competitors revealed that Fork head binding was prevented
by the same single base substitutions that were previously shown to
interfere with the TGTTTGC element function in vivo. Furthermore, the
anti-Fork head antibody bound to >60 sites of polytene chromosomes,
including the puffs of all Sgs genes and Fork head protein was detected in
the nuclei of salivary glands of larvae of all examined stages. These data
provide experimental evidence for the hypothesis that the protein encoded
by the fork head gene is required initially for salivary gland formation
and is utilized subsequently in the control of larval genes specifically
expressed in this organ.
ARTICLES
The Drosophila fork head factor directly controls larval salivary gland- specific expression of the glue protein gene Sgs3
National Institute for Basic Biology, Okazaki, Japan.
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