Nucleic Acids Research, Vol 24, Issue 13 2470-2475, Copyright © 1996 by Oxford University Press
J Woo, RB Meyer Jr and HB Gamper
Modified oligodeoxyribonucleotides (ODNs) that have unique hybridization
properties were designed and synthesized for the first time. These ODNs,
called selective binding complementary ODNs (SBC ODNs), are unable to form
stable hybrids with each other, yet are able to form stable, sequence
specific hybrids with complementary unmodified strands of nucleic acid. To
make SBC ODNs, deoxyguanosine (dG) and deoxycytidine (dC) were substituted
with deoxyinosine (dI) and 3-(2'-
deoxy-beta-D-ribofuranosyl)pyrrolo-[2,3-d]-pyrimidine-2-(3H)-one (dP),
respectively. The hybridization properties of several otherwise identical
complementary ODNs containing one or both of these nucleoside analogs were
studied by both UV monitored thermal denaturation and non- denaturing PAGE.
The data showed that while dI and dP did form base pairs with dC and dG,
respectively, dI did not form a stable base pair with dP. A
self-complementary ODN uniformly substituted with dI and dP acquired
single-stranded character and was able to strand invade the end of a duplex
DNA better than an unsubstituted ODN. This observation implies that SBC
ODNs should effectively hybridize to hairpins present in single-stranded
DNA or RNA.
ARTICLES
G/C-modified oligodeoxynucleotides with selective complementarity: synthesis and hybridization properties
Epoch Pharmaceuticals, Inc., Bothell, WA 98021, USA.
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