Nucleic Acids Research, Vol 24, Issue 13 2519-2524, Copyright © 1996 by Oxford University Press
U Guldener, S Heck, T Fielder, J Beinhauer and JH Hegemann
The dominant kanr marker gene plays an important role in gene disruption
experiments in budding yeast, as this marker can be used in a variety of
yeast strains lacking the conventional yeast markers. We have developed a
loxP-kanMX-loxP gene disruption cassette, which combines the advantages of
the heterologous kanr marker with those from the Cre-lox P recombination
system. This disruption cassette integrates with high efficiency via
homologous integration at the correct genomic locus (routinely 70%). Upon
expression of the Cre recombinase the kanMX module is excised by an
efficient recombination between the loxP sites, leaving behind a single
loxP site at the chromosomal locus. This system allows repeated use of the
kanr marker gene and will be of great advantage for the functional analysis
of gene families.
ARTICLES
A new efficient gene disruption cassette for repeated use in budding yeast
Institut fur Mikrobiologie und Molekularbiologie, Justus-Liebig- Universitat Giessen, Giessen, Germany.
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J. D. Irvin and B. F. Pugh Genome-wide Transcriptional Dependence on TAF1 Functional Domains J. Biol. Chem., March 10, 2006; 281(10): 6404 - 6412. [Abstract] [Full Text] [PDF]
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P. Ternes, P. Sperling, S. Albrecht, S. Franke, J. M. Cregg, D. Warnecke, and E. Heinz Identification of Fungal Sphingolipid C9-methyltransferases by Phylogenetic Profiling J. Biol. Chem., March 3, 2006; 281(9): 5582 - 5592. [Abstract] [Full Text] [PDF]
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A. ZHELKOVSKY, Y. TACAHASHI, T. NASSER, X. HE, U. STERZER, T. H. JENSEN, H. DOMDEY, and C. MOORE The role of the Brr5/Ysh1 C-terminal domain and its homolog Syc1 in mRNA 3'-end processing in Saccharomyces cerevisiae RNA, March 1, 2006; 12(3): 435 - 445. [Abstract] [Full Text] [PDF]
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F. J. van Werven and H. Th. M. Timmers The use of biotin tagging in Saccharomyces cerevisiae improves the sensitivity of chromatin immunoprecipitation Nucleic Acids Res., February 25, 2006; 34(4): e33 - e33. [Abstract] [Full Text] [PDF]
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S. M. G. Saerens, K. J. Verstrepen, S. D. M. Van Laere, A. R. D. Voet, P. Van Dijck, F. R. Delvaux, and J. M. Thevelein The Saccharomyces cerevisiae EHT1 and EEB1 Genes Encode Novel Enzymes with Medium-chain Fatty Acid Ethyl Ester Synthesis and Hydrolysis Capacity J. Biol. Chem., February 17, 2006; 281(7): 4446 - 4456. [Abstract] [Full Text] [PDF]
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Z. Hu, B. Potthoff, C. P. Hollenberg, and M. Ramezani-Rad Mdy2, a ubiquitin-like (UBL)-domain protein, is required for efficient mating in Saccharomyces cerevisiae J. Cell Sci., January 15, 2006; 119(2): 326 - 338. [Abstract] [Full Text] [PDF]
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Y.-S. Jin, H. Alper, Y.-T. Yang, and G. Stephanopoulos Improvement of Xylose Uptake and Ethanol Production in Recombinant Saccharomyces cerevisiae through an Inverse Metabolic Engineering Approach Appl. Envir. Microbiol., December 1, 2005; 71(12): 8249 - 8256. [Abstract] [Full Text] [PDF]
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S. Lebaron, C. Froment, M. Fromont-Racine, J.-C. Rain, B. Monsarrat, M. Caizergues-Ferrer, and Y. Henry The Splicing ATPase Prp43p Is a Component of Multiple Preribosomal Particles Mol. Cell. Biol., November 1, 2005; 25(21): 9269 - 9282. [Abstract] [Full Text] [PDF]
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J. Zhao, J. Herrera-Diaz, and D. S. Gross Domain-Wide Displacement of Histones by Activated Heat Shock Factor Occurs Independently of Swi/Snf and Is Not Correlated with RNA Polymerase II Density Mol. Cell. Biol., October 15, 2005; 25(20): 8985 - 8999. [Abstract] [Full Text] [PDF]
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K. Melcher Mutational Hypersensitivity of a Gene Regulatory Protein: Saccharomyces cerevisiae Gal80p Genetics, October 1, 2005; 171(2): 469 - 476. [Abstract] [Full Text] [PDF]
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K. Meiling-Wesse, U. D. Epple, R. Krick, H. Barth, A. Appelles, C. Voss, E.-L. Eskelinen, and M. Thumm Trs85 (Gsg1), a Component of the TRAPP Complexes, Is Required for the Organization of the Preautophagosomal Structure during Selective Autophagy via the Cvt Pathway J. Biol. Chem., September 30, 2005; 280(39): 33669 - 33678. [Abstract] [Full Text] [PDF]
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J. E. Smotrys, M. J. Schoenfish, M. A. Stutz, and M. E. Linder The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p J. Cell Biol., September 26, 2005; 170(7): 1091 - 1099. [Abstract] [Full Text] [PDF]
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 L. Walsh, P.W. Hastwell, P.O. Keenan, A.W. Knight, N. Billinton, and R.M. Walmsley Genetic modification and variations in solvent increase the sensitivity of the yeast RAD54-GFP genotoxicity assay Mutagenesis, September 1, 2005; 20(5): 317 - 327. [Abstract] [Full Text] [PDF]
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L. Kozubowski, J. R. Larson, and K. Tatchell Role of the Septin Ring in the Asymmetric Localization of Proteins at the Mother-Bud Neck in Saccharomyces cerevisiae Mol. Biol. Cell, August 1, 2005; 16(8): 3455 - 3466. [Abstract] [Full Text] [PDF]
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L. K. Palmer, J. L. Shoemaker, B. A. Baptiste, D. Wolfe, and R. L. Keil Inhibition of Translation Initiation by Volatile Anesthetics Involves Nutrient-sensitive GCN-independent and -dependent Processes in Yeast Mol. Biol. Cell, August 1, 2005; 16(8): 3727 - 3739. [Abstract] [Full Text] [PDF]
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S. Krappmann, O. Bayram, and G. H. Braus Deletion and Allelic Exchange of the Aspergillus fumigatus veA Locus via a Novel Recyclable Marker Module Eukaryot. Cell, July 1, 2005; 4(7): 1298 - 1307. [Abstract] [Full Text] [PDF]
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N. Puri, J. Lai-Zhang, S. Meier, and D. M. Mueller Expression of Bovine F1-ATPase with Functional Complementation in Yeast Saccharomyces cerevisiae J. Biol. Chem., June 10, 2005; 280(23): 22418 - 22424. [Abstract] [Full Text] [PDF]
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J. R. Mullen, F. S. Nallaseth, Y. Q. Lan, C. E. Slagle, and S. J. Brill Yeast Rmi1/Nce4 Controls Genome Stability as a Subunit of the Sgs1-Top3 Complex Mol. Cell. Biol., June 1, 2005; 25(11): 4476 - 4487. [Abstract] [Full Text] [PDF]
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C. Olivier, G. Poirier, P. Gendron, A. Boisgontier, F. Major, and P. Chartrand Identification of a Conserved RNA Motif Essential for She2p Recognition and mRNA Localization to the Yeast Bud Mol. Cell. Biol., June 1, 2005; 25(11): 4752 - 4766. [Abstract] [Full Text] [PDF]
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P. Poulsen, B. Wu, R. F. Gaber, and M. C. Kielland-Brandt Constitutive Signal Transduction by Mutant Ssy5p and Ptr3p Components of the SPS Amino Acid Sensor System in Saccharomyces cerevisiae Eukaryot. Cell, June 1, 2005; 4(6): 1116 - 1124. [Abstract] [Full Text] [PDF]
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 M. DeVit, P. J. Cullen, M. Branson, G. F. Sprague Jr., and S. Fields Forcing interactions as a genetic screen to identify proteins that exert a defined activity Genome Res., April 1, 2005; 15(4): 560 - 565. [Abstract] [Full Text] [PDF]
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V. Pilauri, M. Bewley, C. Diep, and J. Hopper Gal80 Dimerization and the Yeast GAL Gene Switch Genetics, April 1, 2005; 169(4): 1903 - 1914. [Abstract] [Full Text] [PDF]
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