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Nucleic Acids Research, Vol 24, Issue 13 2575-2577, Copyright © 1996 by Oxford University Press

ARTICLES

Obligatory activator-polymerase addition order at promoters

X Zhang and R Schleif
Biology Department, Johns Hopkins University, Baltimore, MD 21218, USA.

The kinetics of open complex formation were measured by migration retardation assay and DNase I footprinting at the activator-dependent promoters ara P1, lac P1 and gal P1. In each case, the rate of open complex formation was significantly faster if the activator, AraC for ara and CAP for lac and gal, had been added before RNA polymerase. The results indicate that complexes of transcriptional activators, RNA polymerase and promoter can exist in two states, one which can form open complexes rapidly and one which cannot.
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[Abstract] [Full Text]


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