Nucleic Acids Research, Vol 24, Issue 14 2690-2696, Copyright © 1996 by Oxford University Press
F Liu and S Altman
M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coli, has
been covalently linked at its 3' terminus to oligonucleotides (guide
sequences) that guide the enzyme to target RNAs through hybridization with
the target sequences. These constructs (M1GS RNAs) have been used to
determine some minimal features of model substrates. As few as 3 bp on the
3' side of the site of cleavage in a substrate complex and 1 nt on the 5'
side are required for cleavage to occur. The cytosines in the 3' terminal
CCA sequence of the model substrates are important for cleavage efficiency
but not cleavage site selection. A purine (base- paired or not) at the 3'
side of the cleavage site is important both for cleavage site selection and
efficiency. M1GS RNAs provide both a simple system for characterization of
the reaction governed by M1 RNA and a tool for gene therapy.
ARTICLES
Requirements for cleavage by a modified RNase P of a small model substrate
Department of Biology, Yale University, New Haven, CT 06520, USA.
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