Nucleic Acids Research, Vol 24, Issue 15 2905-2910, Copyright © 1996 by Oxford University Press
N Liu, FC Lucibello, J Zwicker, K Engeland and R Muller
B-myb belongs to a group of cell cycle genes whose transcription is
repressed in G0/early G1 through a binding site for the transcription
factor E2F. Here, we show that the B-myb repressor element is specifically
recognised by heterodimers consisting of DP-1 and E2F-1, E2F-3 or E2F-4.
Surprisingly, E2F-mediated repression is dependent on a contiguous
corepressor element that resembles the CHR previously established as a
corepressor of the CDE in cell cycle genes derepressed in S/G2, such as
cyclin A, cdc2 and cdc25C. A factor binding to the B- myb CHR was
identified in fractionated HeLa nuclear extract and found to interact with
the minor groove, as previously shown by in vivo footprinting for the
cyclin A CHR. The B-myb and cdc25C CHRs are related with respect to protein
binding but are functionally clearly distinct. Our results support a model
where both E2F- and CDE-mediated repression, acting at different stages in
the cell cycle, are dependent on promoter-specific CHR elements.
ARTICLES
Cell cycle-regulated repression of B-myb transcription: cooperation of an E2F site with a contiguous corepressor element
Institut fur Molekularbiologie und Tumorforschung (IMT), Philipps- Universitat Marburg, Germany.
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