Nucleic Acids Research, Vol 24, Issue 16 3167-3172, Copyright © 1996 by Oxford University Press
F Cecconi, C Crosio, P Mariottini, G Cesareni, M Giorgi, S Brenner and F Amaldi
The compact genome of Fugu rubripes, with its very small introns, appears
to be particularly suitable to study intron-encoded functions. We have
analyzed the Fugu gene for ribosomal protein S7 (formerly S8, see Note),
whose Xenopus homolog contains in its introns the coding sequences for the
small nucleolar RNA U17. Except for intron length, the organization of the
Fugu S7 gene is very similar to that of the Xenopus counterpart. The total
length of the Fugu S7 gene is 3930 bp, compared with 12691 bp for Xenopus.
This length difference is uniquely due to smaller introns. Although short,
the six introns are longer than the approximately 100 bp size of most Fugu
introns, as they host U17 RNA coding sequences. While four of the six U17
sequences are 'canonical', the remaining two represent diverged U17
pseudocopies. In fact, microinjection in Xenopus oocytes of in vitro
synthesized Fugu transcripts containing the 'canonical' U17f sequence
results in efficient production of mature U17 RNA, while injection of a
transcript containing the U17 psi b sequence does not.
ARTICLES
A functional role for some Fugu introns larger than the typical short ones: the example of the gene coding for ribosomal protein S7 and snoRNA U17
Dipartimento di Biologia, Universita di Roma Tor Vergata, Rome, Italy.
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