Nucleic Acids Research, Vol 24, Issue 16 3208-3215, Copyright © 1996 by Oxford University Press
VY Stefanovsky, DP Bazett-Jones, G Pelletier and T Moss
The formation of a near complete loop of DNA is a striking property of the
architectural HMG-box factor xUBF. Here we show that DNA looping only
requires a dimer of Nbox13, a C-terminal truncation mutant of xUBF
containing just HMG-boxes 1-3. This segment of xUBF corresponds to that
minimally required for activation of polymerase I transcription and is
sufficient to generate the major characteristics of the footprint given by
intact xUBF. Stepwise reduction in the number of HMG-boxes to less than
three significantly diminishes DNA bending and provides an estimate of bend
angle for each HMG-box. Together the data indicate that a 350 +/- 16 degree
loop in 142 +/- 30 bp of DNA can be induced by binding of the six HMG-boxes
in an Nbox13 dimer and that DNA looping is probably achieved by six
in-phase bends. The positioning of each HMG- box on the DNA does not
predominantly involve DNA sequence recognition and is thus an intrinsic
property of xUBF.
ARTICLES
The DNA supercoiling architecture induced by the transcription factor xUBF requires three of its five HMG-boxes
Departement de Biochimie et Centre de Recherche en Cancerologie de l'Universite Laval (CRCUL), Hotel-Dieu de Quebec, Canada.
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