Nucleic Acids Research, Vol 24, Issue 16 3229-3234, Copyright © 1996 by Oxford University Press
L Marechal-Drouard, R Kumar, C Remacle and I Small
Larch mitochondria contain a'native'tRNAHis which is absent from
angiosperms. Sequence comparisons of genomic DNA and cDNA obtained from
unprocessed primary transcripts of the larch mitochondrial gene trnH
encoding this tRNA revealed three nucleotide discrepancies. These three
nucleotide alterations, in the acceptor stem, D stem and anticodon stem
respectively, are conversions of genomic cytidines to thymidines in the
cDNA (uridines in the tRNA) and thus resemble the RNA editing events
observed in nearly all plant mitochondrial mRNAs. Two cases of editing
affecting mitochondrial tRNAs from angiosperms have already been described,
but we present here the first example of such events in a gymnosperm
mitochondrial tRNA. All three editing events correct mismatched C x A base
pairs which appear when folding the gene sequence into the standard
cloverleaf structure, thereby improving the secondary structure of the
tRNA. When incubated with a heterologous potato mitochondrial processing
extract, only the edited form of the larch mitochondrial tRNAHis precursor
was efficiently processed in vitro. These data strongly suggest that
editing of larch mitochondrial tRNAHis is a prerequisite for its
processing.
ARTICLES
RNA editing of larch mitochondrial tRNA(His) precursors is a prerequisite for processing
Institut de Biologie Moleculaire des Plantes du CNRS, Universite Louis Pasteur, Strasbourg, France.
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