Nucleic Acids Research, Vol 24, Issue 16 3276-3277, Copyright © 1996 by Oxford University Press
B Seraphin and S Kandels-Lewis
We describe here an improved megaprimer PCR mutagenesis strategy. The
cumbersome gel purification step that is usually used can be omitted by
appropriately cleaving the first and second DNA templates with restriction
enzymes and enzymatically removing remaining primers from the first PCR
reaction. We show that this improved procedure is reproducible and highly
efficient. Furthermore this method is suitable for automation because all
the steps are now carried out in reaction tubes.
ARTICLES
An efficient PCR mutagenesis strategy without gel purification [correction of purificiation] step that is amenable to automation
EMBL, Heidelberg, Germany.
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