Nucleic Acids Research, Vol 24, Issue 17 3289-3294, Copyright © 1996 by Oxford University Press
S Kumar, M Giacca, P Norio, G Biamonti, S Riva and A Falaschi
In the past, a highly sensitive and efficient method was developed to map
DNA replication origins in human cells, based on quantitative PCR performed
on nascent DNA samples. This method allowed the identification of a
replication origin in the myeloid HL-60 cell line, located on chromosome 19
within an approximately 500 bp segment near the lamin B2 gene [Giacca et
al. (1994) Proc. Natl. Acad. Sci. USA, 91, 7119]. The same procedure has
now been further simplified and extended to a variety of other
exponentially growing human cells of different histological derivation
(three neural, one connectival and one epithelial), with a nearly diploid
chromosomal content. In all the six cell lines tested, the origin activity
within the lamin B2 gene domain was localized to the same region.
Furthermore, the lamin B2 origin was also found to be active in stimulated,
but not in quiescent, peripheral blood lymphocytes.
ARTICLES
Utilization of the same DNA replication origin by human cells of different derivation
International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
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