Nucleic Acids Research, Vol 24, Issue 17 3307-3312, Copyright © 1996 by Oxford University Press
T Roldan-Arjona, C Anselmino and T Lindahl
The Escherichia coli endonuclease III (Nth-Eco) protein is involved in the
removal of damaged pyrimidine residues from DNA by base excision repair. It
is an iron-sulphur enzyme possessing both DNA glycosylase and
apurinic/apyrimidinic lyase activities. A database homology search
identified an open reading frame in genomic sequences of
Schizosaccharomyces pombe which encodes a protein highly similar to Nth-
Eco. The gene has been subcloned in an expression vector and the protein
purified to apparent homogeneity. The S.pombe Nth homologue (Nth-Spo) is a
40.2 kDa protein of 355 amino acids. Nth-Spo possesses glycosylase activity
on different types of DNA substrates with pyrimidine damage, being able to
release both urea and thymine glycol from double-stranded polymers. The
eukaryotic protein removes urea more efficiently than the prokaryotic
enzyme, whereas its efficiency in excising thymine glycol is lower. A
nicking assay was used to show that the enzyme also exhibits an AP lyase
activity on UV- and gamma- irradiated DNA substrates. These findings show
that Nth protein is structurally and functionally conserved from bacteria
to fission yeast.
ARTICLES
Molecular cloning and functional analysis of a Schizosaccharomyces pombe homologue of Escherichia coli endonuclease III
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, UK.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Watanabe, J. O. Blaisdell, S. S. Wallace, and J. P. Bond Engineering Functional Changes in Escherichia coli Endonuclease III Based on Phylogenetic and Structural Analyses J. Biol. Chem., October 7, 2005; 280(40): 34378 - 34384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Denver, S. L. Swenson, and M. Lynch An Evolutionary Analysis of the Helix-Hairpin-Helix Superfamily of DNA Repair Glycosylases Mol. Biol. Evol., October 1, 2003; 20(10): 1603 - 1611. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Matsumoto, Q.-M. Zhang, M. Takao, A. Yasui, and S. Yonei Escherichia coli Nth and human hNTH1 DNA glycosylases are involved in removal of 8-oxoguanine from 8-oxoguanine/guanine mispairs in DNA Nucleic Acids Res., May 1, 2001; 29(9): 1975 - 1981. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yang, S. Fitz-Gibbon, E. M. Marcotte, J. H. Tai, E. C. Hyman, and J. H. Miller Characterization of a Thermostable DNA Glycosylase Specific for U/G and T/G Mismatches from the Hyperthermophilic Archaeon Pyrobaculum aerophilum J. Bacteriol., March 1, 2000; 182(5): 1272 - 1279. [Abstract] [Full Text]
|
|
|
|
|
|
A. Masaoka, H. Terato, M. Kobayashi, A. Honsho, Y. Ohyama, and H. Ide Enzymatic Repair of 5-Formyluracil. I. EXCISION OF 5-FORMYLURACIL SITE-SPECIFICALLY INCORPORATED INTO OLIGONUCLEOTIDE SUBSTRATES BY AlkA PROTEIN (Escherichia coli 3-METHYLADENINE DNA GLYCOSYLASE II) J. Biol. Chem., August 27, 1999; 274(35): 25136 - 25143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Alseth, L. Eide, M. Pirovano, T. Rognes, E. Seeberg, and M. Bjoras The Saccharomyces cerevisiae Homologues of Endonuclease III from Escherichia coli, Ntg1 and Ntg2, Are Both Required for Efficient Repair of Spontaneous and Induced Oxidative DNA Damage in Yeast Mol. Cell. Biol., May 1, 1999; 19(5): 3779 - 3787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Stierum, D. L. Croteau, and V. A. Bohr Purification and Characterization of a Mitochondrial Thymine Glycol Endonuclease from Rat Liver J. Biol. Chem., March 12, 1999; 274(11): 7128 - 7136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. O. Bennett The Saccharomyces cerevisiae ETH1 Gene, an Inducible Homolog of Exonuclease III That Provides Resistance to DNA-Damaging Agents and Limits Spontaneous Mutagenesis Mol. Cell. Biol., March 1, 1999; 19(3): 1800 - 1809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ikeda, T. Biswas, R. Roy, T. Izumi, I. Boldogh, A. Kurosky, A. H. Sarker, S. Seki, and S. Mitra Purification and Characterization of Human NTH1, a Homolog of Escherichia coli Endonuclease III. DIRECT IDENTIFICATION OF LYS-212 AS THE ACTIVE NUCLEOPHILIC RESIDUE J. Biol. Chem., August 21, 1998; 273(34): 21585 - 21593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Jiang, Z. Hatahet, R. J. Melamede, Y. W. Kow, and S. S. Wallace Characterization of Escherichia coli Endonuclease VIII J. Biol. Chem., December 19, 1997; 272(51): 32230 - 32239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Roldan-Arjona, Y.-F. Wei, K. C. Carter, A. Klungland, C. Anselmino, R.-P. Wang, M. Augustus, and T. Lindahl Molecular cloning and functional expression of a human cDNA encoding the antimutator enzyme 8-hydroxyguanine-DNA glycosylase PNAS, July 22, 1997; 94(15): 8016 - 8020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Aspinwall, D. G. Rothwell, T. Roldan-Arjona, C. Anselmino, C. J. Ward, J. P. Cheadle, J. R. Sampson, T. Lindahl, P. C. Harris, and I. D. Hickson Cloning and characterization of a functional human homolog of Escherichia coli endonuclease III PNAS, January 7, 1997; 94(1): 109 - 114. [Abstract] [Full Text] [PDF]
|
|