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Nucleic Acids Research, Vol 24, Issue 17 3431-3436, Copyright © 1996 by Oxford University Press

ARTICLES

In vivo analysis of the plasmid pAM beta 1 resolution system

L Janniere, S McGovern, C Pujol, MA Petit and SD Ehrlich
Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.

The promiscuous plasmid pAM beta 1 from Gram-positive bacteria encodes a resolution system which differs from that of Tn3 in that (i) it requires a histone-like protein and an unusual resolvase-DNA interaction to promote recombination and (ii) it mediates in vivo DNA inversion in plasmid substrates. In this in vivo analysis, the pAM beta 1 resolution site is narrowed down to a 99 bp segment, the strand exchange is mapped within 10 bp and the serine residue at position 10 of the resolvase is shown to be essential for enzyme activity. In addition, data showing that the resolution system does not promote DNA inversion in the Bacillus subtilis chromosome are presented. Implications of this observation are discussed.
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