Nucleic Acids Research, Vol 24, Issue 18 3499-3506, Copyright © 1996 by Oxford University Press
M Tijsterman, JG Tasseron-de Jong, P van de Putte and J Brouwer
Repair of UV-induced cyclobutane pyrimidine dimers (CPDs) was examined at
single nucleotide resolution in the yeast Saccharomyces cerevisiae, using
an improved protocol for genomic end-labelling. To obtain the sensitivity
required for adduct detection in yeast, an oligonucleotide- directed
enrichment step was introduced into the current methodology developed for
adduct detection in Escherichia coli. With this method, heterogeneous
repair of CPDs within the RPB2 locus is observed. Individual CPDs
positioned in the transcribed strand are removed very efficiently with
identical kinetics. This fast repair starts within 23 bases downstream of
the transcription initiation site. The non- transcribed strand of the
active gene exhibits slow repair without detectable repair variations
between individual lesions. In contrast, CPDs positioned in the promoter
region show profound repair heterogeneity. Here, CPDs at specific sites are
removed very quickly, with comparable rates to CPDs positioned in the
transcribed strand, while at other positions lesions are not repaired at
all during the period studied. Interestingly, the fast repair in the
promoter region is dependent on the RAD7 and RAD16 genes, as are the slowly
repaired CPDs in this region and in the non-transcribed strand. This
indicates that the global genome repair pathway is not intrinsically slow
and at specific positions can be as efficient as the transcription-coupled
repair pathway.
ARTICLES
Transcription-coupled and global genome repair in the Saccharomyces cerevisiae RPB2 gene at nucleotide resolution
Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, The Netherlands.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Q. Svejstrup Rescue of arrested RNA polymerase II complexes J. Cell Sci., February 1, 2003; 116(3): 447 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Martinez, V. B. Palhan, A. Tjernberg, E. S. Lymar, A. M. Gamper, T. K. Kundu, B. T. Chait, and R. G. Roeder Human STAGA Complex Is a Chromatin-Acetylating Transcription Coactivator That Interacts with Pre-mRNA Splicing and DNA Damage-Binding Factors In Vivo Mol. Cell. Biol., October 15, 2001; 21(20): 6782 - 6795. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yasuhira, M. Morimyo, and A. Yasui Transcription Dependence and the Roles of Two Excision Repair Pathways for UV Damage in Fission Yeast Schizosaccharomyces pombe J. Biol. Chem., September 17, 1999; 274(38): 26822 - 26827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tijsterman and J. Brouwer Rad26, the Yeast Homolog of the Cockayne Syndrome B Gene Product, Counteracts Inhibition of DNA Repair Due to RNA Polymerase II Transcription J. Biol. Chem., January 15, 1999; 274(3): 1199 - 1202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tijsterman, R. de Pril, J. G. Tasseron-de Jong, and J. Brouwer RNA Polymerase II Transcription Suppresses Nucleosomal Modulation of UV-Induced (6-4) Photoproduct and Cyclobutane Pyrimidine Dimer Repair in Yeast Mol. Cell. Biol., January 1, 1999; 19(1): 934 - 940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tu, S. Bates, and G. P. Pfeifer Sequence-specific and Domain-specific DNA Repair in Xeroderma Pigmentosum and Cockayne Syndrome Cells J. Biol. Chem., August 15, 1997; 272(33): 20747 - 20755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tijsterman, R. A. Verhage, P. van de Putte, J. G. T.-d. Jong, and J. Brouwer Transitions in the coupling of transcription and nucleotide excision repair within RNA polymerase II-transcribed genes of Saccharomyces cerevisiae PNAS, July 22, 1997; 94(15): 8027 - 8032. [Abstract] [Full Text] [PDF]
|
|