Nucleic Acids Research, Vol 24, Issue 18 3514-3521, Copyright © 1996 by Oxford University Press
KE Plant, A Hair and GT Morgan
We have identified cDNAs encoding three related forms of transcription
elongation factor TFIIS (S-II) in Xenopus laevis ovary. Comparison of
Xenopus and mammalian sequences identifies likely diagnostic amino acids
that distinguish classes of vertebrate TFIIS. The diversity of TFIIS
polypeptides in Xenopus is due partly to the presence of two diverged genes
in this tetraploid genome. We isolated genomic clones containing one of the
genes, xTFIIS.oA, and, unlike a previously described vertebrate TFIIS gene,
found that it contains introns. Alternative splicing at a CAG/CAG motif
containing the 3' splice site of intron 4 produces the third form of
xTFIIS, which differs from one of the others simply in lacking Ser109.
Intron 6 of xTFIIS.oA contains splice and branch site consensus sequences
conforming to those of the minor class of AT-AC introns and this was
confirmed for the homeologous xTFIIS.oB gene by genomic PCR. Other unusual
but functional variants of RNA processing signals were found in xTFIIS
genes at the 5' splice site of intron 8 and the polyadenylation
hexanucleotides. Utilization of multiple unusual processing signals may
make the generation of mature xTFIIS.o mRNAs inefficient and the possible
regulatory consequences of this are discussed.
ARTICLES
Genes encoding isoforms of transcription elongation factor TFIIS in Xenopus and the use of multiple unusual RNA processing signals
Department of Genetics, University of Nottingham, UK.
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