Nucleic Acids Research, Vol 24, Issue 18 3533-3537, Copyright © 1996 by Oxford University Press
MP Longhese, H Neecke, V Paciotti, G Lucchini and P Plevani
The rfa1-M2 and rfa1-M4 Saccharomyces cerevisiae mutants, which are altered
in the 70 kDa subunit of replication protein A (RPA) and sensitive to UV
and methyl methane sulfonate (MMS), have been analyzed for possible
checkpoint defects. The G1/S and intra-S DNA damage checkpoints are
defective in the rfa1-M2 mutant, since rfa1-M2 cells fail to properly delay
cell cycle progression in response to UV irradiation in G1 and MMS
treatment during S phase. Conversely, the G2/M DNA damage checkpoint and
the S/M checkpoint are proficient in rfa1-M2 cells and all the checkpoints
tested are functional in the rfa1- M4 mutant. Preventing S phase entry by
alpha-factor treatment after UV irradiation in G1 does not change rfa1-M4
cell lethality, while it allows partial recovery of rfa1-M2 cell viability.
Therefore, the hypersensitivity to UV and MMS treatments observed in the
rfa1-M4 mutant might only be due to impairment of RPA function in DNA
repair, while the rfa1-M2 mutation seems to affect both the DNA repair and
checkpoint functions of Rpa70.
ARTICLES
The 70 kDa subunit of replication protein A is required for the G1/S and intra-S DNA damage checkpoints in budding yeast
Dipartimento di Genetica e di Biologia dei Microrganismi, Universita di Milano, Italy.
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