Nucleic Acids Research, Vol 24, Issue 18 3576-3582, Copyright © 1996 by Oxford University Press
MS Satoh and PC Hanawalt
In mammalian cells, mRNA transcription is initiated with the aid of
transcription initiation factors. Of these, TFIIH has also been shown to
play an essential role in nucleotide excision repair (NER), which is a
versatile biochemical pathway that corrects a broad range of DNA damage.
Since the dual role of TFIIH is conserved among eukaryotes, including yeast
and mammalian cells, the sharing of TFIIH between NER and RNA transcription
initiation might provide some survival advantage. However, the functional
relationship between NER and RNA transcription initiation through TFIIH is
not yet understood. We have developed an optimized cell-free assay which
allows us to analyze NER and RNA transcription under identical conditions.
In this assay, NER did not compete with RNA transcription, probably because
the extracts contained sufficient amounts of TFIIH to support both
processes. Thus, NER can be considered functionally independent of RNA
transcription initiation despite the fact that both processes use the same
factor.
ARTICLES
TFIIH-mediated nucleotide excision repair and initiation of mRNA transcription in an optimized cell-free DNA repair and RNA transcription assay
Department of Biological Sciences, Stanford University, CA 94305-5020, USA.
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