Nucleic Acids Research, Vol 24, Issue 19 3797-3805, Copyright © 1996 by Oxford University Press
X Gu and WF Marzluff
The highly expressed mouse histone H2a-614 gene is located 800 nt 5' of the
histone H3-614 gene. There is a 140 nt sequence located 500 nt from the end
of the H2-614 mRNA which has been defined as a transcription termination
site for RNA polymerase II. We established an in vitro transcription system
in which both 3' end processing and transcription termination occur. A
template containing the adenovirus major late promoter, a portion of the
histone H2a-614 coding region, its 3' processing signal, followed by the
transcription termination site was transcribed in a nuclear extract
prepared from mouse myeloma cells. Some of the transcripts synthesized in
the extract were cleaved at the histone processing site in a reaction which
was dependent both on the hairpin binding factor and the U7 snRNP. The
efficiency of histone 3' end formation was similar both on synthetic
transcripts and transcripts synthesized by RNA polymerase II. Defined
transcripts, which were not processed and which mapped to the transcription
termination site, were released from the template, suggesting that they
were formed by transcription termination. Termination in vitro was
dependent on a functional histone processing signal.
ARTICLES
3' Processing and termination of mouse histone transcripts synthesized in vitro by RNA polymerase II
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, 27599, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Richard and J. L. Manley Transcription termination by nuclear RNA polymerases Genes & Dev., June 1, 2009; 23(11): 1247 - 1269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nag, K. Narsinh, A. Kazerouninia, and H. G. Martinson The conserved AAUAAA hexamer of the poly(A) signal can act alone to trigger a stable decrease in RNA polymerase II transcription velocity RNA, August 1, 2006; 12(8): 1534 - 1544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Park, D. C. Tsao, and H. G. Martinson The Two Steps of Poly(A)-Dependent Termination, Pausing and Release, Can Be Uncoupled by Truncation of the RNA Polymerase II Carboxyl-Terminal Repeat Domain Mol. Cell. Biol., May 15, 2004; 24(10): 4092 - 4103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Kim and H. G. Martinson Poly(A)-dependent Transcription Termination: CONTINUED COMMUNICATION OF THE POLY(A) SIGNAL WITH THE POLYMERASE IS REQUIRED LONG AFTER EXTRUSION IN VIVO J. Biol. Chem., October 24, 2003; 278(43): 41691 - 41701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhao, L. Hyman, and C. Moore Formation of mRNA 3' Ends in Eukaryotes: Mechanism, Regulation, and Interrelationships with Other Steps in mRNA Synthesis Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 405 - 445. [Abstract] [Full Text] [PDF]
|
|