Nucleic Acids Research, Vol 24, Issue 2 257-263, Copyright © 1996 by Oxford University Press
J Schmitz, D Prufer, W Rohde and E Tacke
The 5' untranslated leader (Omega sequence) of tobacco mosaic virus (TMV)
genomic RNA was utilized as a translational enhancer sequence in expression
of the 17 kDa putative movement protein (pr17) of potato leaf roll
luteovirus (PLRV). In vitro translation of RNAs transcribed from
appropriate chimeric constructs, as well as their expression in transgenic
potato plants, resulted in the expected wild-type pr17 protein, as well as
in larger translational products recognized by pr17- specific antisera.
Mutational analyses revealed that the extra proteins were translated by
non-canonical initiation at AUU codons present in the wild-type Omega
sequence. In the plant system translation initiated predominantly at the
AUU codon at positions 63-65 of the Omega sequence. Additional AUU codons
in a different reading frame of the Omega sequence also showed the capacity
for efficient translation initiation in vitro. These results extend the
previously noted activity of the TMV 5' leader sequence in ribosome binding
and translation enhancement in that the TMV translation enhancer can
mediate non- canonical translation initiation in vitro and in vivo.
ARTICLES
Non-canonical translation mechanisms in plants: efficient in vitro and in planta initiation at AUU codons of the tobacco mosaic virus enhancer sequence
MPI fur Zuchtungsforschung, Koln, Germany.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  I. P. Ivanov, G. Loughran, and J. F. Atkins uORFs with unusual translational start codons autoregulate expression of eukaryotic ornithine decarboxylase homologs PNAS, July 22, 2008; 105(29): 10079 - 10084. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Hu, H.-C. Fu, C.-H. Lin, H.-J. Su, and H.-H. Yeh Reassortment and Concerted Evolution in Banana Bunchy Top Virus Genomes J. Virol., February 15, 2007; 81(4): 1746 - 1761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Di Fiore, Q. Li, M. J. Leech, F. Schuster, N. Emans, R. Fischer, and S. Schillberg Targeting Tryptophan Decarboxylase to Selected Subcellular Compartments of Tobacco Plants Affects Enzyme Stability and in Vivo Function and Leads to a Lesion-Mimic Phenotype Plant Physiology, July 1, 2002; 129(3): 1160 - 1169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Riechmann, T. Ito, and E. M. Meyerowitz Non-AUG Initiation of AGAMOUS mRNA Translation in Arabidopsis thaliana Mol. Cell. Biol., December 1, 1999; 19(12): 8505 - 8512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Vaquero, M. Sack, J. Chandler, J. Drossard, F. Schuster, M. Monecke, S. Schillberg, and R. Fischer Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves PNAS, September 28, 1999; 96(20): 11128 - 11133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, S. Tong, and J. R. Wands Identification and Expression of Glycine Decarboxylase (p120) as a Duck Hepatitis B Virus Pre-S Envelope-binding Protein J. Biol. Chem., September 24, 1999; 274(39): 27658 - 27665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sasaki and N. Nakashima Translation Initiation at the CUU Codon Is Mediated by the Internal Ribosome Entry Site of an Insect Picorna-Like Virus In Vitro J. Virol., February 1, 1999; 73(2): 1219 - 1226. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Shirako Non-AUG Translation Initiation in a Plant RNA Virus: a Forty-Amino-Acid Extension Is Added to the N Terminus of the Soil-Borne Wheat Mosaic Virus Capsid Protein J. Virol., February 1, 1998; 72(2): 1677 - 1682. [Abstract] [Full Text] [PDF]
|
|