Nucleic Acids Research, Vol 24, Issue 2 380-385, Copyright © 1996 by Oxford University Press
J Cheng, MA Shoffner, GE Hvichia, LJ Kricka and P Wilding
We examined PCR in silicon dioxide-coated silicon-glass chips (12 microl in
volume with a surface to volume ratio of approximately 17.5 mm(2)/microl)
using two PCR reagent systems: (i) the conventional reagent system using
Taq DNA polymerase; (ii) the hot-start reagent system based on a mixture of
TaqStart antibody and Taq DNA polymerase. Quantitative results obtained
from capillary electrophoresis for the expected amplification products
showed that amplification in microchips was reproducible (between batch
coefficient of variation 7.71%) and provided excellent yields. We also used
the chip for PCR directly from isolated intact human lymphocytes. The
amplification results were comparable with those obtained using extracted
human genomic DNA. This investigation is fundamental to the integration of
sample preparation, polynucleotide amplification and amplicate detection on
a microchip.
ARTICLES
Chip PCR. II. Investigation of different PCR amplification systems in microbabricated silicon-glass chips
Department of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania, Philadelphia, 19104, USA.
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