Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (150K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (66)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Newman, S.
Right arrow Articles by Butow, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Newman, S.
Right arrow Articles by Butow, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 24, Issue 2 386-393, Copyright © 1996 by Oxford University Press

ARTICLES

Analysis of mitochondrial DNA nucleoids in wild-type and a mutant strain of Saccharomyces cerevisiae that lacks the mitochondrial HMG box protein Abf2p

SM Newman, O Zelenaya-Troitskaya, PS Perlman and RA Butow
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, 75235, USA.

DNA-protein complexes (nucleoids) are believed to be the segregating unit of mitochondrial DNA (mtDNA) in Saccharomyces cerevisiae. A mitochondrial HMG box protein, Abf2p, is needed for maintenance of mtDNA in cells grown on rich dextrose medium, but is dispensible in glycerol grown cells. As visualized by 4',6'-diamino-2-phenylindole staining, mtDNA nucleoids in mutant cells lacking Abf2p ( delta abf2) are diffuse compared with those in wild-type cells. We have isolated mtDNA nucleoids and characterized two mtDNA-protein complexes, termed NCLDp-2 and NCLDs-2, containing distinct but overlapping sets of polypeptides. This protocol yields similar nucleoid complexes from the delta abf2 mutant, although several proteins appear lacking from NCLDs- 2. Segments of mtDNA detected with probes to COXII, VAR1 and ori5 sequences are equally sensitive to DNase I digestion in NCLDs-2 and NCLDp-2 from wild-type cells and from the delta abf2 mutant. However, COXII and VAR1 sequences are 4-to 5-fold more sensitive to DNase I digestion of mtDNA in toluene-permeabilized mitochondria from the delta abf2 mutant than from wild-type cells, but no difference in DNase I sensitivity was detected with the ori5 probe. These results provide a first indication that Abf2p influences differential organization of mtDNA sequences.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
R. A. Sia, S. Carrol, L. Kalifa, C. Hochmuth, and E. A. Sia
Loss of the Mitochondrial Nucleoid Protein, Abf2p, Destabilizes Repetitive DNA in the Yeast Mitochondrial Genome
Genetics, January 1, 2009; 181(1): 331 - 334.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Kucej, B. Kucejova, R. Subramanian, X. J. Chen, and R. A. Butow
Mitochondrial nucleoids undergo remodeling in response to metabolic cues
J. Cell Sci., June 1, 2008; 121(11): 1861 - 1868.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Dai, Y.-S. Lo, A. Litvinchuk, Y.-T. Wang, W.-N. Jane, L.-J. Hsiao, and K.-S. Chiang
Structural and functional characterizations of mung bean mitochondrial nucleoids
Nucleic Acids Res., August 22, 2005; 33(15): 4725 - 4739.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. D. Taylor, H. Zhang, J. S. Eaton, M. S. Rodeheffer, M. A. Lebedeva, T. W. O'Rourke, W. Siede, and G. S. Shadel
The Conserved Mec1/Rad53 Nuclear Checkpoint Pathway Regulates Mitochondrial DNA Copy Number in Saccharomyces cerevisiae
Mol. Biol. Cell, June 1, 2005; 16(6): 3010 - 3018.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
K. S. Dimmer, S. Jakobs, F. Vogel, K. Altmann, and B. Westermann
Mdm31 and Mdm32 are inner membrane proteins required for maintenance of mitochondrial shape and stability of mitochondrial DNA nucleoids in yeast
J. Cell Biol., January 3, 2005; 168(1): 103 - 115.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. J. Youngman, A. E. A. Hobbs, S. M. Burgess, M. Srinivasan, and R. E. Jensen
Mmm2p, a mitochondrial outer membrane protein required for yeast mitochondrial shape and maintenance of mtDNA nucleoids
J. Cell Biol., March 1, 2004; 164(5): 677 - 688.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
D. F. Bogenhagen, Y. Wang, E. L. Shen, and R. Kobayashi
Protein Components of Mitochondrial DNA Nucleoids in Higher Eukaryotes
Mol. Cell. Proteomics, November 1, 2003; 2(11): 1205 - 1216.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Garrido, L. Griparic, E. Jokitalo, J. Wartiovaara, A. M. van der Bliek, and J. N. Spelbrink
Composition and Dynamics of Human Mitochondrial Nucleoids
Mol. Biol. Cell, April 1, 2003; 14(4): 1583 - 1596.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. I. Alam, T. Kanki, T. Muta, K. Ukaji, Y. Abe, H. Nakayama, K. Takio, N. Hamasaki, and D. Kang
Human mitochondrial DNA is packaged with TFAM
Nucleic Acids Res., March 15, 2003; 31(6): 1640 - 1645.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Bateman, M. Iacovino, P. S. Perlman, and R. A. Butow
Mitochondrial DNA Instability Mutants of the Bifunctional Protein Ilv5p Have Altered Organization in Mitochondria and Are Targeted for Degradation by Hsp78 and the Pim1p Protease
J. Biol. Chem., November 27, 2002; 277(49): 47946 - 47953.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. Dequard-Chablat and C. Alland
Two Copies of mthmg1, Encoding a Novel Mitochondrial HMG-Like Protein, Delay Accumulation of Mitochondrial DNA Deletions in Podospora anserina
Eukaryot. Cell, August 1, 2002; 1(4): 503 - 513.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. A. Karlok, S.-H. Jang, and J. A. Jaehning
Mutations in the Yeast Mitochondrial RNA Polymerase Specificity Factor, Mtf1, Verify an Essential Role in Promoter Utilization
J. Biol. Chem., July 26, 2002; 277(31): 28143 - 28149.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. M. Bateman, P. S. Perlman, and R. A. Butow
Mutational Bisection of the Mitochondrial DNA Stability and Amino Acid Biosynthetic Functions of Ilv5p of Budding Yeast
Genetics, July 1, 2002; 161(3): 1043 - 1052.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. W. O'Rourke, N. A. Doudican, M. D. Mackereth, P. W. Doetsch, and G. S. Shadel
Mitochondrial Dysfunction Due to Oxidative Mitochondrial DNA Damage Is Reduced through Cooperative Actions of Diverse Proteins
Mol. Cell. Biol., June 15, 2002; 22(12): 4086 - 4093.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
X. M. Zuo, G. D. Clark-Walker, and X. J. Chen
The Mitochondrial Nucleoid Protein, Mgm101p, of Saccharomyces cerevisiae Is Involved in the Maintenance of {rho}+ and ori/rep-Devoid Petite Genomes but Is Not Required for Hypersuppressive {rho}- mtDNA
Genetics, April 1, 2002; 160(4): 1389 - 1400.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. E. A. Hobbs, M. Srinivasan, J. M. McCaffery, and R. E. Jensen
Mmm1p, a Mitochondrial Outer Membrane Protein, Is Connected to Mitochondrial DNA (Mtdna) Nucleoids and Required for Mtdna Stability
J. Cell Biol., January 22, 2001; 152(2): 401 - 410.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Tomaska, J. Nosek, A. M. Makhov, A. Pastorakova, and J. D. Griffith
Extragenomic double-stranded DNA circles in yeast with linear mitochondrial genomes: potential involvement in telomere maintenance
Nucleic Acids Res., November 15, 2000; 28(22): 4479 - 4487.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. Oxelmark, A. Marchini, I. Malanchi, F. Magherini, L. Jaquet, M. A. N. Hajibagheri, K. J. Blight, J.-C. Jauniaux, and M. Tommasino
Mmf1p, a Novel Yeast Mitochondrial Protein Conserved throughout Evolution and Involved in Maintenance of the Mitochondrial Genome
Mol. Cell. Biol., October 15, 2000; 20(20): 7784 - 7797.
[Abstract] [Full Text]

Home page
 Mol. Biol. CellHome page
Y. Tang, G. Manfredi, M. Hirano, and E. A. Schon
Maintenance of Human Rearranged Mitochondrial DNAs in Long-Term Cultured Transmitochondrial Cell Lines
Mol. Biol. Cell, July 1, 2000; 11(7): 2349 - 2358.
[Abstract] [Full Text]

Home page
 Microbiol. Mol. Biol. Rev.Home page
V. Contamine and M. Picard
Maintenance and Integrity of the Mitochondrial Genome: a Plethora of Nuclear Genes in the Budding Yeast
Microbiol. Mol. Biol. Rev., June 1, 2000; 64(2): 281 - 315.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. K. Lehtinen, N. Hance, A. El Meziane, M. K. Juhola, K. M. I. Juhola, R. Karhu, J. N. Spelbrink, I. J. Holt, and H. T. Jacobs
Genotypic Stability, Segregation and Selection in Heteroplasmic Human Cell Lines Containing np 3243 Mutant mtDNA
Genetics, January 1, 2000; 154(1): 363 - 380.
[Abstract] [Full Text]

Home page
 JCBHome page
S. Meeusen, Q. Tieu, E. Wong, E. Weiss, D. Schieltz, J. R. Yates, and J. Nunnari
Mgm101p Is a Novel Component of the Mitochondrial Nucleoid That Binds DNA and Is Required for the Repair of Oxidatively Damaged Mitochondrial DNA
J. Cell Biol., April 19, 1999; 145(2): 291 - 304.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. H. Cho, S. J. Ha, L. R. Kao, T. L. Megraw, and C.-B. Chae
A Novel DNA-Binding Protein Bound to the Mitochondrial Inner Membrane Restores the Null Mutation of Mitochondrial Histone Abf2p in Saccharomyces cerevisiae
Mol. Cell. Biol., October 1, 1998; 18(10): 5712 - 5723.
[Abstract] [Full Text]

Home page
 JCBHome page
K. Okamoto, P. S. Perlman, and R. A. Butow
The Sorting of Mitochondrial DNA and Mitochondrial Proteins in Zygotes: Preferential Transmission of Mitochondrial DNA to the Medial Bud
J. Cell Biol., August 10, 1998; 142(3): 613 - 623.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. M. MacAlpine, P. S. Perlman, and R. A. Butow
The high mobility group protein Abf2p influences the level of yeast mitochondrial DNA recombination intermediates in vivo
PNAS, June 9, 1998; 95(12): 6739 - 6743.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
O. Zelenaya-Troitskaya, S. M. Newman, K. Okamoto, P. S. Perlman, and R. A. Butow
Functions of the High Mobility Group Protein, Abf2p, in Mitochondrial DNA Segregation, Recombination and Copy Number in Saccharomyces cerevisiae
Genetics, April 1, 1998; 148(4): 1763 - 1776.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. A. Kaufman, S. M. Newman, R. L. Hallberg, C. A. Slaughter, P. S. Perlman, and R. A. Butow
In organello formaldehyde crosslinking of proteins to mtDNA: Identification of bifunctional proteins
PNAS, July 5, 2000; 97(14): 7772 - 7777.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.