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Nucleic Acids Research, Vol 24, Issue 20 4003-4008, Copyright © 1996 by Oxford University Press

ARTICLES

Molecular cloning of the cDNA encoding a murine sialic acid-specific 9- O-acetylesterase and RNA expression in cells of hematopoietic and non- hematopoietic origin

A Stoddart, Y Zhang and CJ Paige
The Wellesley Hospital Research Institute, University of Toronto, Ontario, Canada.

We describe the isolation of a cDNA encoding a murine sialic acid- specific 9-O-acetylesterase as well as its expression pattern in cells of both hematopoietic and non-hematopoietic origin. This enzyme catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid N-acetylneuraminic acid. The cDNA is 2105 nt in length and encodes a protein of 541 amino acids with a predicted molecular weight of 61 kDa, not including oligosaccharides linked to eight potential N-glycosylation sites. The cDNA encoding the acetylesterase displays a widespread distribution in various cell lines and tissues. Expression studies of B lineage cell lines and primary fetal liver cells revealed a developmentally regulated expression pattern in cells of hematopoietic origin. Given the importance of 9-O- acetylation of sialic acids, the cloning of the cDNA encoding a sialic acid-specific 9-O-acetylesterase will be helpful in understanding further the regulation of this post-translational modification and the biological consequences thereof.
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