Nucleic Acids Research, Vol 24, Issue 20 4009-4014, Copyright © 1996 by Oxford University Press
M Grey, A Dusterhoft, JA Henriques and M Brendel
The radiation-sensitive mutant pso4-1 of Saccharomyces cerevisiae shows a
pleiotropic phenotype, including sensitivity to DNA cross-linking agents,
nearly blocked sporulation and reduced mutability. We have cloned the
putative yeast DNA repair gene PSO4 from a genomic library by
complementation of the blocked UV-induced mutagenesis and of sporulation in
diploids homozygous for pso4-1. Sequence analysis revealed that gene PSO4
consists of 1512 bp located upstream of UBI4 on chromosome XII and encodes
a putative protein of 56.7 kDa. PSO4 is allelic to PRP19, a gene encoding a
spliceosome-associated protein, but shares no significant homology with
other yeast genes. Gene disruption with a destroyed reading frame of our
PSO4 clone resulted in death of haploid cells, confirming the finding that
PSO4/PRP19 is an essential gene. Thus, PSO4 is the third essential DNA
repair gene found in the yeast S.cerevisiae.
ARTICLES
Allelism of PSO4 and PRP19 links pre-mRNA processing with recombination and error-prone DNA repair in Saccharomyces cerevisiae
Institut fur Mikrobiologie der J.W.Goethe Universitat, Frankfurt/Main,Germany.
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