Nucleic Acids Research, Vol 24, Issue 20 4092-4093, Copyright © 1996 by Oxford University Press
BA Eldadah, AG Yakovlev and AI Faden
Apoptotic cell death is often characterized by internucleosomal cleavage of
genomic DNA, which exhibits a distinctive ladder upon electrophoresis.
However, techniques used for the isolation and detection of DNA to
demonstrate laddering may not be sufficiently sensitive, particularly when
cleaved DNA is present at modest levels. We propose a new approach for
isolating total cellular DNA using a silica-based resin that improves the
resolution of DNA laddering. In addition, we introduce a rapid DNA labeling
method that can increase the sensitivity of detecting DNA laddering. Each
of these methods can be used for DNA from cell cultures or tissues.
ARTICLES
A new approach for the electrophoretic detection of apoptosis
Department of Neurology and the Georgetown Institute for Cognitive and Computational Sciences, Georgetown University Medical Center, Washington, DC 20007, USA.
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