Nucleic Acids Research, Vol 24, Issue 21 4165-4170, Copyright © 1996 by Oxford University Press
DY Chang, K Hsu and RJ Maraia
Nearly 1 000 000 copies of Alu interspersed elements comprise approximately
5% of human DNA. Alu elements cause gene disruptions by a process known as
retrotransposition, in which dimeric Alu RNA is a presumed intermediate.
Dimeric Alu transcripts are labile, giving rise to stable left monomeric
scAlu RNAs whose levels are tightly regulated. Induction of Alu RNA by
viral infection or cell stress leads to a dramatic increase in dimeric Alu
transcripts, while scAlu RNA increases modestly. Each monomer of the
dimeric Alu element shares sequence homology with the 7SL RNA component of
the signal recognition particle (SRP). The SRP protein known as SRP9/14 is
also found in a discrete complex with scAlu RNA, although whether dimeric
Alu RNA is associated with SRP9/14 had been unknown. Here we show that
antiserum to human SRP9 immunoprecipitates both scAlu RNA and dimeric Alu
RNAs and that these RNPs accumulate after adenovirus infection, while
levels of SRP9, SRP14, SRP54 and 7SL SRP RNA are unaffected. Dimeric Alu
RNAs are also associated with the La protein, indicating that these are
indeed nascent RNA polymerase III transcripts. This report documents that
induced Alu transcripts are assembled into SRP9/14-containing RNPs in vivo
while SRP levels are unchanged. Implications for Alu RNA metabolism and
evolution are discussed.
ARTICLES
Monomeric scAlu and nascent dimeric Alu RNAs induced by adenovirus are assembled into SRP9/14-containing RNPs in HeLa cells
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
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